SubtiBank SubtiBank
ansA [Mon Aug 31 2015 13:55:25 GMT+0200 (CEST)]
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ansA [Mon Aug 31 2015 13:55:25 GMT+0200 (CEST)]

L-asparaginase
locus
BSU23580
pI
4.46
mw
36.00 kDa
function
asparagine degradation
product
L-asparaginase
essential
no
ec
3.5.1.1
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.6|Utilization of asparagine/ aspartate]
  • [SW|Categories] containing this gene/protein

  • [SW|utilization of amino acids]
  • This gene is a member of the following [SW|regulons]

  • [SW|AnsR regulon]
  • The protein

    Catalyzed reaction/ biological activity

  • L-asparagine + H2O = L-aspartate + NH3 (according to Swiss-Prot)
  • Protein family

  • asparaginase 1 family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|search|AnsZ]
  • Expression and Regulation

    Operon

  • ''[protein|search|ansA]-[protein|search|ansB]'' [Pubmed|1711029]
  • ''[protein|search|ansA]-[protein|search|ansB]-[protein|search|mleN]-[protein|search|mleA]'' [Pubmed|22383849]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|1711029]
  • Regulation

  • expressed in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]
  • Regulatory mechanism

  • [protein|search|AnsR]: transcription repression [Pubmed|11914346]
  • Biological materials

    Mutant

  • GP1153 (del ''ansAB''::''ermC'') available in the [SW|Stülke] lab
  • lacZ fusion

  • pGP872 (in [protein|search|pAC5]), available in [SW|Stülke] lab
  • References

  • 8478318,1711029,11914346,22383849