SubtiBank SubtiBank
leuS
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

leuS

Leu-tRNA synthetase
locus
BSU30320
pI
4.86
mw
91.35 kDa
protein length
804 aa Sequence Blast
gene length
2412 bp Sequence Blast
function
translation
product
leucyl-tRNA synthetase
essential
yes
ec
6.1.1.4
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    3,102,629 → 3,105,043

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu) (according to Swiss-Prot)
  • Protein family

  • class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Structure

  • [PDB|2AJG] (from ''Escherichia coli'', 39% identity, 56% similarity) [Pubmed|16277600]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|FEC89C6F5A825D925D730965ACBAA06EC5CC4E44|leuS]
    description
    [Pubmed|1317842]

    regulatory mechanism

  • [regulon|T-box|T-box]: transcription antitermination, via uncharged tRNA-Leu binding to [SW|RNA switch], in [regulon|T-box|T-box]
  • regulation

  • induced by leucine limitation ([protein|search|T-box]) [Pubmed|1317842]
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    References

    Reviews

  • 19258532
  • Original Publications

  • 1317842