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gsiB [2018-06-21 16:05:11]
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gsiB [2018-06-21 16:05:11]

general stress protein, prevents enzyme inactivation upon freeze-thaw treatments
locus
BSU04400
pI
5.14
mw
13.66 kDa
protein length
123 aa Sequence Blast
gene length
369 bp Sequence Blast
function
response to water deficits
product
general stress protein
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

    • Gene

    Coordinates
    494,506 → 494,877

    The protein

    Structure

  • [PDB|3JQH] (human glycan binding receptor, 31% identity) [pubmed|19835887]

    • Expression and Regulation

    Operons

    genes
    [gene|FE56282883B3229D3D6836A1BE7EA4292D3CC799|gsiB]
    description
    [Pubmed|1378051]

    sigma factors

  • [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI]: sigma factor, [Pubmed|11157964], in [regulon|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

    • regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|11544224]

    • additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
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    Biological materials

    Mutant

  • 1A735 ( ''gsiB''::''kan''), [Pubmed|1378051], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A735&Search=1A735 BGSC]
  • BKE04400 (Δ[gene|FE56282883B3229D3D6836A1BE7EA4292D3CC799|gsiB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGAATTCCTCCTTTA, downstream forward: _UP4_TAATCAAAAGCATAAAGGCA
  • BKK04400 (Δ[gene|FE56282883B3229D3D6836A1BE7EA4292D3CC799|gsiB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGAATTCCTCCTTTA, downstream forward: _UP4_TAATCAAAAGCATAAAGGCA

    • References

  • 9669336,12884008,7651322,11544224,1378051,21421783,23861025,11918677,19835887