SubtiBank SubtiBank
nrdEB
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nrdEB

similar to ribonucleoside-diphosphate reductase (alpha subunit)
locus
BSU_20060
pI
6.39
mw
27.82 kDa
protein length
1168 aa Sequence Blast
gene length
3507 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
yosN
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.5|Biosynthesis/ acquisition of nucleotides/ other]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.2|SP-beta prophage]

    • Gene

    Coordinates
    2,162,108 → 2,165,614

    The protein

    Catalyzed reaction/ biological activity

  • [thioredoxin]-disulfide + 2'-deoxyribonucleoside 5'-diphosphate + H2O --> [thioredoxin]-dithiol + ribonucleoside 5'-diphosphate (according to UniProt)

    • Protein family

  • ribonucleoside diphosphate reductase large chain family (with [protein|3B68CE8E9A35997B45E437A316996D585E43B76B|NrdE], according to UniProt)

    • Paralogous protein(s)

  • [protein|3B68CE8E9A35997B45E437A316996D585E43B76B|NrdE]

    • [SW|Domains]

  • DOD-type homing endonuclease domain (aa 503-654) (according to UniProt)

    • Biological materials

    Mutant

  • BKE20060 (Δ[gene|FD4CD7DB35AA20DBBEC0A4DA5D88282D89CCDA6D|nrdEB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE20060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTGAGCTTGATCCAA, downstream forward: _UP4_TAATAGGTACGTTTACGTTT
  • BKK20060 (Δ[gene|FD4CD7DB35AA20DBBEC0A4DA5D88282D89CCDA6D|nrdEB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK20060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTGAGCTTGATCCAA, downstream forward: _UP4_TAATAGGTACGTTTACGTTT

    • References

  • 21498918,11470879,16621400,9465078