polC [Fri Dec 23 2016 15:42:37 GMT+0100 (CET)]

DNA polymerase III (alpha subunit), part of the replisome

locus: BSU16580

pI: 5.00

mw: 162.00 kDa

function: DNA replication

product: DNA polymerase III (alpha subunit)

essential: yes

ec: 2.7.7.7

synonyms: mutI, dnaF, dnaP

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|DNA replication], [SW|essential genes]

Gene

Coordinates on the chromosome (coding sequence): 1,727,133 -> 1,731,446

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• Deoxynucleoside triphosphate DNA(n) = diphosphate DNA(n1) (according to Swiss-Prot)
• required for bacteriophage SPP1 replication [Pubmed|23268446]

Protein family

• PolC subfamily (according to Swiss-Prot)

Structure

• [PDB|3F2B] (from ''Geobacillus kaustophilus'', 71% identity) [Pubmed|19106298]

[SW|Localization]

• forms foci [Pubmed|21958350]
• cytoplasm (according to Swiss-Prot)

[SW|Interactions]

• part of the [SW|replisome]: [protein|search|PolC]-[protein|search|HolA]-[protein|search|HolB]-[protein|search|DnaX]-[protein|search|DnaN]-[protein|search|DnaG]-[protein|search|DnaC]-[protein|search|DnaI]-[protein|search|DnaD]-[protein|search|SsbA]-[protein|search|DnaE]-[protein|search|PriA]-[protein|search|DnaB] [Pubmed|20122408]
• [protein|search|DnaE]-[protein|search|PolC] [Pubmed|23563155]

Expression and Regulation

Additional information

• there are about 60 molecules of PolC present per cell [Pubmed|28002733]

References

Reviews

• 19106294

Original publications

• 15186423,20122408,11721055,23563155,23268446,21740522,21958350,24062730,24106089,19106298,28002733