SubtiBank SubtiBank
polC
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polC

DNA polymerase III, leading strand polymerase (alpha subunit), part of the [SW|replisome]
locus
BSU_16580
pI
5.14
mw
162.42 kDa
protein length
1437 aa Sequence Blast
gene length
4311 bp Sequence Blast
function
[category|SW 3.1.1|DNA replication]
product
DNA polymerase III (alpha subunit)
essential
yes
ec
2.7.7.7
synonyms
mutI,dnaF,dnaP

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    1,727,133 → 1,731,446

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
  • required for bacteriophage SPP1 replication [Pubmed|23268446]
  • Protein family

  • DNA polymerase type-C family (with [protein|48BC0FA9901F174472EB070C539EC75EC7C10E88|YorL] and [protein|65B5EA4DBDF5B260DED754B8A7B226AA88F17B55|DnaE], according to UniProt)
  • [SW|Domains]

  • [SW|Exonuclease domain] (aa 421-578) (according to UniProt)
  • Structure

  • [PDB|3F2B] (from ''Geobacillus kaustophilus'', 71% identity) [Pubmed|19106298]
  • [SW|Localization]

  • forms foci [Pubmed|21958350]
  • about three molecules of PolC at each replication fork [pubmed|28002733]
  • Expression and Regulation

    Operons

    genes
    [gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC]-[gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP]-[gene|848570563D3C92A8E1E6AB4DA530388D8AE94E75|proS]-[gene|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC]
    description
    [Pubmed|22383849]
    view in new tab

    genes
    [gene|4A637EB9B400D30E5C6246D1E0EB5E6A30F38A16|uppS]-[gene|7E7F6BBA03C443A5AFA05A6D9352FEF1212A5AF6|cdsA]-[gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC]-[gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP]-[gene|848570563D3C92A8E1E6AB4DA530388D8AE94E75|proS]-[gene|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC]
    description
    [Pubmed|22383849]
    view in new tab

    additional information

  • about 60 molecules per cell [pubmed|28002733]
  • References

    Reviews

  • 19106294,28073967,29197672
  • Original publications

  • 15186423,20122408,11721055,23563155,23268446,21740522,21958350,24062730,24106089,19106298,28002733,28575448,28878042,30686488,31251806