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opuAA [Sun Aug 21 2016 20:50:25 GMT+0200 (CEST)]
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opuAA [Sun Aug 21 2016 20:50:25 GMT+0200 (CEST)]

glycine betaine ABC transporter (ATP-binding protein)
locus
BSU02980
pI
5.00
mw
46.00 kDa
function
compatible solute transport
product
glycine betaine ABC transporter (ATP-binding protein)
essential
no
ec
3.6.3.32
synonyms

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.4|Uptake of compatible solutes for osmoprotection]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|ABC transporters], [SW|coping with hyper-osmotic stress], [SW|membrane proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|RemA regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    321,013 -> 322,269

    The protein

    Catalyzed reaction/ biological activity

  • uptake of glycine betaine
  • uptake of dimethylglycine [Pubmed|24561588]
  • Protein family

  • [SW|ABC transporter] domain (according to Swiss-Prot)
  • [SW|Domains]

  • 2 [SW|CBS domain]s (284-340, 344-403)
  • [SW|Localization]

  • associated to the membrane (via [SW|OpuAB]) [Pubmed|10092453]
  • [SW|Interactions]

  • [SW|OpuAA]-[SW|OpuAB]-[SW|OpuAC] [Pubmed|10092453]
  • Expression and Regulation

    Operon

  • ''[SW|opuAA]-[SW|opuAB]-[SW|opuAC]'' [Pubmed|22383849]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|23175650]
  • Regulation

  • induced by osmotic stress [Pubmed|23175650]
  • repressed by intracellular glycine betaine [Pubmed|23175650]
  • Regulatory mechanism

  • [protein|search|RemA]: transcription activation [Pubmed|23646920]
  • Biological materials

    Expression vector

  • pGP2924 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]
  • References

  • 7622480,10092453,16645306,14623183,18321243,16225868,21296969,22383849,23175650,23646920,24561588