SubtiBank SubtiBank
yqhY [2017-06-07 11:45:27]
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yqhY [2017-06-07 11:45:27]

unknown
locus
BSU24330
pI
4.41
mw
14.55 kDa
protein length
135 aa Sequence Blast
gene length
405 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.13|Quasi-essential genes]
  • Gene

    Coordinates
    2,529,926 → 2,530,333

    The protein

    Protein family

  • asp23 family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|YloU]
  • Expression and Regulation

    Operons

    genes
    [gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB]-[gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC]-[gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|yqhY]
    description
    [Pubmed|7592499]

    regulation

  • expression of the operon is strongly reduced during [SW|biofilm formation] [pubmed|31113899]
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    Biological materials

    Mutant

  • MGNA-C367 (yqhY::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2365 NBRP B. subtilis, Japan]
  • GP1468 (''yqhY''::''erm''), available in [SW|Jörg Stülke]'s lab [Pubmed|24178028]
  • GP1765 (''yqhY''::''cat''), available in [SW|Jörg Stülke]'s lab [Pubmed|24178028]
  • Expression vector

  • GP1474 (chromosomal ''yqhY''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • pGP1322 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • pGP1325 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP1496 (His-tag, purification from ''E. coli'', in pET28a+), available in [SW|Jörg Stülke]'s lab
  • pGP1498 (N-terminal His-tag, TEV-site, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • GFP fusion

  • GP1471 (spc, based on [SW|pBP43]), available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP1481 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • References

  • 17114254,7592499,23420519,24178028