SubtiBank SubtiBank
yqhY [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]
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yqhY [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

unknown
locus
BSU24330
pI
4.41
mw
14.00 kDa
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.13|Quasi-essential genes]
  • [SW|Categories] containing this gene/protein

  • [SW|proteins of unknown function]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,529,926 -> 2,530,333

    The protein

    Protein family

  • asp23 family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|search|YloU]
  • Expression and Regulation

    Operon

  • ''[protein|search|accB]-[protein|search|accC]-[protein|search|yqhY]'' [Pubmed|7592499]
  • Biological materials

    Mutant

  • GP1468 (''yqhY''::''erm''), available in [SW|Jörg Stülke]'s lab [Pubmed|24178028]
  • GP1765 (''yqhY''::''cat''), available in [SW|Jörg Stülke]'s lab [Pubmed|24178028]
  • Expression vector

  • GP1474 (chromosomal ''yqhY''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • pGP1322 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • pGP1325 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP1496 (His-tag, purification from ''E. coli'', in pET28a+), available in [SW|Jörg Stülke]'s lab
  • pGP1498 (N-terminal His-tag, TEV-site, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • GFP fusion

  • GP1471 (spc, based on [SW|pBP43]), available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP1481 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • References

  • 17114254,7592499,23420519,24178028