SubtiBank SubtiBank
gatB
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

gatB

production of glutamyl-tRNA(Gln)
locus
BSU06690
pI
4.90
mw
53.24 kDa
protein length
476 aa Sequence Blast
gene length
1428 bp Sequence Blast
function
translation
product
glutamyl-tRNA(Gln) amidotransferase (sununit B)
essential
yes
ec
6.3.5.7
synonyms
yerN

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    730,509 → 731,939

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP L-glutamyl-tRNA(Gln) L-glutamine = ADP phosphate L-glutaminyl-tRNA(Gln) L-glutamate (according to Swiss-Prot)
  • Protein family

  • GatB subfamily (according to Swiss-Prot)
  • Structure

  • [PDB|2DF4] ([protein|FA88576A405F8C92C83BE74985039DC02E1F6C94|GatA]-[protein|F9C088D2DB3E99FDF961DE1B78A9B81279F204C9|GatB]-[protein|4A6AB40A91B4821B99E2FECD1C4E523321B8BDEA|GatC] complex from ''Staphylococcus aureus'') [Pubmed|16809541]
  • Expression and Regulation

    Operons

    genes
    [gene|4A6AB40A91B4821B99E2FECD1C4E523321B8BDEA|gatC]-[gene|FA88576A405F8C92C83BE74985039DC02E1F6C94|gatA]-[gene|F9C088D2DB3E99FDF961DE1B78A9B81279F204C9|gatB]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B447 (yerN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1446 NBRP B. subtilis, Japan]
  • References

  • 9342321,20882017,16809541