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gatB

production of glutamyl-tRNA(Gln)

locus: BSU_06690

pI: 4.90

mw: 53.24 kDa

protein length: 476 aa

gene length: 1431 bp

function: translation

product: glutamyl-tRNA(Gln) amidotransferase (sununit B)

essential: yes

ec: 6.3.5.7

synonyms: yerN

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 730,509 → 731,939

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-glutamine + L-glutamyl-tRNA^Gln --> ADP + H⁺ + L-glutamate + L-glutaminyl-tRNA^Gln + phosphate (according to UniProt)
• ATP + H₂O + L-aspartyl-tRNA^Asn + L-glutamine --> ADP + 2 H⁺ + L-asparaginyl-tRNA^Asn + L-glutamate + phosphate (according to UniProt)

Protein family

• GatB/GatE family (single member, according to UniProt)

Structure

• [PDB|2DF4] ([protein|FA88576A405F8C92C83BE74985039DC02E1F6C94|GatA]-[protein|F9C088D2DB3E99FDF961DE1B78A9B81279F204C9|GatB]-[protein|4A6AB40A91B4821B99E2FECD1C4E523321B8BDEA|GatC] complex from ''Staphylococcus aureus'') [Pubmed|16809541]

Expression and Regulation

Operons

• genes: [gene|4A6AB40A91B4821B99E2FECD1C4E523321B8BDEA|gatC]-[gene|FA88576A405F8C92C83BE74985039DC02E1F6C94|gatA]-[gene|F9C088D2DB3E99FDF961DE1B78A9B81279F204C9|gatB]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B447 (yerN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1446 NBRP B. subtilis, Japan]

References

• 9342321,20882017,16809541