SubtiBank SubtiBank
rplK [2019-06-07 17:08:37]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

rplK [2019-06-07 17:08:37]

[category|search|ribosomal protein]
locus
BSU01020
pI
9.72
mw
14.78 kDa
protein length
141 aa Sequence Blast
gene length
426 bp Sequence Blast
function
translation
product
[category|search|ribosomal protein] L11 (BL11)
essential
no
synonyms
relC,tsp

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]
  • Gene

    Coordinates
    118,591 → 119,016

    Phenotypes of a mutant

  • poor growth [pubmed|28189581]
  • non-transformable [pubmed|28189581]
  • The protein

    Protein family

  • Universal ribosomal protein uL11 family (single member, according to UniProt)
  • Modification

  • phosphorylated on Arg-94 [Pubmed|22517742]
  • Structure

  • [PDB|1FOX] (C-terminal domain, Geobacillus stearothermophilus)
  • [PDB|2FOW] (RNA binding domain in complex with RNA, Geobacillus stearothermophilus)
  • [PDB|3J9W] (the [SW|ribosome]) [Pubmed|25903689]
  • [SW|Localization]

  • [SW|ribosome] (according to UniProt)
  • Expression and Regulation

    Operons

    genes
    [gene|F94A1C14912F35ADB4C611AA17D55268CC26160D|rplK]-[gene|44D4CE353E1E532664A508CBE8B49AC8BB861B53|rplA]-[gene|E4D89640D0F0A31A2814D20FB5425FDB0B46A43D|BSU_misc_RNA_3]-[gene|5EF3A7953AA97D2B44C967542A53FEA7F36D7BA3|rplJ]-[gene|E72910604D3DC2A62AE8A951F55570EA2838B250|rplL]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]
  • regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • view in new tab

    Biological materials

    Mutant

  • BKE01020 (Δ[gene|F94A1C14912F35ADB4C611AA17D55268CC26160D|rplK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAGACACACCTCCTTAA, downstream forward: _UP4_TAATTTGTTTCTTGTCGGGT
  • BKK01020 (Δ[gene|F94A1C14912F35ADB4C611AA17D55268CC26160D|rplK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAGACACACCTCCTTAA, downstream forward: _UP4_TAATTTGTTTCTTGTCGGGT
  • References

  • 11244072,11948165,19653700,20441189,20525796,22517742,112097,23002217,25903689,28516784,28189581