fadM

fatty acid degradation, similar to proline dehydrogenase

Locus: BSU_32850

Isoelectric point: 9.38

Molecular weight: 34.30 kDa

Protein length: 302 aa

Gene length: 909 bp

Function: fatty acid degradation

Product: unknown

Essential: no

Synonyms: yusM

Genomic Context

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Categories containing this gene/protein

• Metabolism → Lipid metabolism → Utilization of lipids → Utilization of fatty acids
• Lifestyles → Sporulation → Sporulation proteins → Newly identified sporulation proteins (based on transcription profiling)

Gene

Coordinates: 3,372,740 → 3,373,648

The protein

Catalyzed reaction/ biological activity

• quinone + L-proline --> (S)-1-pyrroline-5-carboxylate + quinol + H⁺ (according to UniProt)

Protein family

• proline dehydrogenase family (with PutB, according to UniProt)

Paralogous protein(s)

• PutB

Cofactors

• FAD (according to UniProt)

Structure

• 4H6Q (from Deinococcus radiodurans, 48% identity) PubMed

Expression and Regulation

Operons

• Genes: fadM-fadN-fadA-fadE
  Description: PubMed

  Regulatory mechanism

  • FadR: repression, PubMed, in FadR regulon
  • SdpR: activation, PubMed, in SdpR regulon

  Regulation

  • fadM: induced by long-chain fatty acids (FadR) PubMed

Biological materials

Mutant

• MGNA-A598 (yusM::erm), available at the NBRP B. subtilis, Japan
• BKE32850 (ΔfadM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGACTCCCTCCCGCCG, downstream forward: _UP4_TGAAAGCAGCCGTGGGAAGC
• BKK32850 (ΔfadM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGACTCCCTCCCGCCG, downstream forward: _UP4_TGAAAGCAGCCGTGGGAAGC

References

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