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fur [2017-10-05 09:06:43]
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fur [2017-10-05 09:06:43]

transcription regulator of iron homoeostasis, sensor of Fe sufficiency
locus
BSU23520
pI
5.37
mw
17.26 kDa
protein length
149 aa Sequence Blast
gene length
447 bp Sequence Blast
function
regulation of iron homoeostasis
product
transcriptional repressor Fur family
essential
no
synonyms
yqkL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.1|Acquisition of iron / Other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

    • Gene

    Coordinates
    2,449,841 → 2,450,290

    Phenotypes of a mutant

  • no growth with glucose and ammonium as single sources of carbon and nitrogen, respectively (due to [protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|FsrA]-mediated repression of the ''[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]'' operon) [Pubmed|22389480]
  • poor frowth on lactate as single carbon source (due to overexpression of [protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|FsrA]-mediated repression of the ''[gene|FEC83B66CEA311EA1650D61A215F82E9DF4E9F03|lutA]-[gene|57B9E37F6232189CD47C1B41FDCF43FCB8016AEB|lutB]-[gene|5EE69198882DF4E9AB8D9D7267F071EF88C2F16D|lutC]'' operon, can be suppressed by inactivation of ''[gene|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|fsrA]'' or ''[gene|147AFEBEA546FDBEEB08E9A8D9C7BCDC9B83CC90|fbpB]'') [Pubmed|22427629]
  • transcription profile of a ''[gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE27416 GEO] [Pubmed|22389480]

    • The protein

    Protein family

  • [SW|Fur family]

    • Paralogous protein(s)

  • [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR], [protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]

    • [SW|Cofactors]

  • Fe(II)

    • Effectors of protein activity

  • DNA binding activity (repression) is triggered by binding of Fe(II) [Pubmed|23057863]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]
    description
    [Pubmed|12029044]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029044], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR]: repression, [Pubmed|12029044], in [regulon|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR regulon]

    • regulation

  • repressed in the absence of hydrogen peroxide ([protein|search|PerR]) [Pubmed|12029044]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C426 (yqkL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2424 NBRP B. subtilis, Japan]
  • HB6543 (aphA3), available in the [SW|John Helmann] lab; also available in the [SW|Stülke] lab GP879 (''fur::mls'') and GP868 (''fur::mls'', ''perR::spc'')
  • 1A910 ( ''fur''::''kan''), [Pubmed|12029044], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A910&Search=1A910 BGSC]
  • BKE23520 (Δ[gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCCCTCCTACGC, downstream forward: _UP4_TAGACGGTGCCGAGCGCGAA
  • BKK23520 (Δ[gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCCCTCCTACGC, downstream forward: _UP4_TAGACGGTGCCGAGCGCGAA

    • Labs working on this gene/protein

  • [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

    • References

    Reviews

  • 15802251,25209494,25160631

    • The [SW|Fur regulon]

  • 12374814,12354229,22389480,21873409

    • Other original publications

  • 18487332,14563870,18697947,10400588,11790741,12207695,9701813,12029044,22427629,17725565,17012385,12950915,19508286,16672620,23057863,25486128,28439033