dnaG [2017-06-05 16:00:37]

DNA primase, part of the replisome

Locus: BSU25210

Isoelectric point: 6.71

Molecular weight: 68.62 kDa

Protein length: 603 aa

Gene length: 1809 bp

Function: DNA replication

Product: DNA primase

Essential: yes

E.C.: 2.7.7.-

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA replication
• Information processing → Targets of second messengers → Targets of (p)ppGpp
• Groups of genes → Essential genes

Gene

Coordinates: 2,601,528 → 2,603,339

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• required for bacteriophage SPP1 replication PubMed

Protein family

• DNA primase family (according to Swiss-Prot)

Domains

• TOPRIM domain (aa 262-343) PubMed

Effectors of protein activity

• the enzymatic activity is inhibited by (p)ppGpp during the 'stringent response' PubMed
• DnaG primase activity is stimulates by DnaC PubMed

Structure

• 1D0Q (zinc binding domain, Geobacillus stearothermophilus)
• 1Z8S (DnaB binding domain, AA 452-597, Geobacillus stearothermophilus)
• 4M4W (the DnaC-DnaI-DnaG complex) PubMed

Localization

• Cytoplasm (Homogeneous) PubMed

Expression and Regulation

Operons

• Genes: yqxD-dnaG-sigA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigH: sigma factor, PubMed, in SigH regulon

  Regulatory mechanism

  • Spo0A: repression, PubMed, in Spo0A regulon

  Regulation

  • enzymatic activity is inhibited by (p)ppGpp during the 'stringent response

  Additional information

  • An antisense RNA is predicted for sigA PubMed

References

Reviews

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Original publications

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