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pyk [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]
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pyk [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

pyruvate kinase, glycolytic enzyme
locus
BSU29180
pI
4.00
mw
61.00 kDa
function
catabolic enzyme in glycolysis
product
pyruvate kinase
essential
no
ec
2.7.1.40
synonyms
pykA

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • [SW|Categories] containing this gene/protein

  • [SW|ATP synthesis], [SW|carbon core metabolism], [SW|phosphoproteins], [SW|most abundant proteins]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,984,788 -> 2,986,545

    Phenotypes of a mutant

  • unable to grow with non-[SW|PTS] carbohydrates (such as glucitol or glycerol) as single carbon source
  • suppression of ''[protein|search|ftsZ]''(ts) mutation (reverted by addition of pyruvate) [Pubmed|24825009]
  • The protein

    Catalyzed reaction/ biological activity

  • ADP phosphoenolpyruvate --> ATP pyruvate
  • The reaction is irreversible under physiological conditions
  • Protein family

  • PEP-utilizing enzyme family (according to Swiss-Prot) pyruvate kinase family, (C-terminal section: PEP-utilizing enzyme family)
  • Modification

  • phosphorylation on Ser36 [Pubmed|17218307], [Pubmed|16493705], phosphorylation on Ser536 or Ser546 [Pubmed|17726680], please note that the Ser is not on position 536 but rather at 538
  • [SW|Cofactors]

  • Mg2, K
  • Effectors of protein activity

  • Activated by PEP (Hill Coefficient 1,8) [Pubmed|4623707] [Pubmed|3711058]
  • Allosterically activated by AMP [Pubmed|3711058]
  • Activation by r5p and ADP [Pubmed|3711058]
  • Inhibition by ADP and f16bp in high concentrations; and ATP [Pubmed|3711058]
  • Structure

  • [PDB|2E28] (Geobacillus stearothermophilus)
  • [SW|Localization]

  • cytoplasm [Pubmed|16479537]
  • Additional information

  • The enzyme is a tetramer with four active sites [Pubmed|3711058]
  • Expression and Regulation

    Operon

  • ''[protein|search|pfkA]-[protein|search|pyk]-[protein|search|ytzA]'' [Pubmed|11489127]
  • Regulation

  • twofold induced by glucose [Pubmed|11489127]
  • Additional information

  • belongs to the 100 [[most abundant proteins]] [PubMed|15378759]
  • Biological materials

    Mutant

  • GP589 (''pyk''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP600 (''pyk''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP1745: ''pyk::aphA3'', available in [SW|Jörg Stülke]' lab
  • Expression vector

  • expression in ''E. coli'', N-terminal His-tag: pGP1100 (in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • expression in ''B. subtilis'', native protein: pGP1411 (in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • expression in ''B. subtilis'', N-terminal Strep-tag: pGP1409 (in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
  • expression in ''B. subtilis'', C-terminal Strep-tag: pGP1410 (in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab
  • lacZ fusion

  • see ''[protein|search|pfkA]
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • References

  • 17726680,16493705,11489127,17505547,10966427,17218307,3711058,4623707,21821766,22846916,23420519,24158146,24571712,15378759,24825009