ycgA

unknown

locus: BSU_03020

pI: 8.24

mw: 41.37 kDa

protein length: 477 aa

gene length: 1434 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 325,339 → 326,772

The protein

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|F71FBBE4436FBC4221C3631794C73D306CD3FC7E|ycgA]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]) [Pubmed|12618455]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• MGNA-B987 (ycgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1986 NBRP B. subtilis, Japan]
• BKE03020 (Δ[gene|F71FBBE4436FBC4221C3631794C73D306CD3FC7E|ycgA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCGATCCATCCCCT, downstream forward: _UP4_TAACGATTGCTGCCCGCCGG
• BKK03020 (Δ[gene|F71FBBE4436FBC4221C3631794C73D306CD3FC7E|ycgA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCGATCCATCCCCT, downstream forward: _UP4_TAACGATTGCTGCCCGCCGG

References

• 12884008,12618455,12618455