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lplJ [2019-05-09 09:57:35]
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lplJ [2019-05-09 09:57:35]

lipoate-protein ligase
locus
BSU10250
pI
5.84
mw
37.86 kDa
protein length
331 aa Sequence Blast
gene length
996 bp Sequence Blast
function
lipoic acid metabolism
product
Lipoate:protein ligase
essential
no
synonyms
yhfJ

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.7|Biosynthesis and scavenging of lipoic acid]
  • Gene

    Coordinates
    1,099,159 → 1,100,154

    The protein

    Structure

  • [PDB|5IBY] (from Enterococcus faecalis, 59% identity)
  • Expression and Regulation

    Operons

    genes
    [gene|8FA225FA26DB0B89812A835EB928F2C8892EBC89|yhfI]-[gene|F68611DF326880FCCB4EE3C2B364ECCC3DEAD78D|lplJ]-[gene|2DA0641FA6EC81072DA585D65FE17B7E5A010AC9|yhfK]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B276 (yhfJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1275 NBRP B. subtilis, Japan]
  • BKE10250 (Δ[gene|F68611DF326880FCCB4EE3C2B364ECCC3DEAD78D|lplJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATAAATAACATGGTGCTCC, downstream forward: _UP4_TAAGGCAAAACACATCGTTT
  • BKK10250 (Δ[gene|F68611DF326880FCCB4EE3C2B364ECCC3DEAD78D|lplJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATAAATAACATGGTGCTCC, downstream forward: _UP4_TAAGGCAAAACACATCGTTT
  • References

    Reviews

  • 27074917
  • Original publications

  • 21338420,21338421