pksG

involved in polyketide synthesis

locus: BSU_17150

pI: 5.14

mw: 46.62 kDa

protein length: 420 aa

gene length: 1263 bp

function: polyketide synthesis

product: hydroxymethylglutaryl CoA synthase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

Gene

Coordinates: 1,789,943 → 1,791,205

The protein

Catalyzed reaction/ biological activity

• 3-oxobutanoyl-[ACP] + acetyl-[ACP] + H₂O --> (3S)-hydroxy-3-methylglutaryl-[ACP] + H⁺ + holo-[ACP] (according to UniProt)

Protein family

• [SW|thiolase-like superfamily] (according to UniProt)

Structure

• [PDB|4YXQ]
• [PDB|4YXT] (E82A mutant)
• [PDB|4YXV] (C114A mutant)

Expression and Regulation

Operons

• genes: [gene|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC]-[gene|F3E9CA9330394FD829D59B0C2D4D1EEDD2C22D3B|pksD]-[gene|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE]-[gene|6BEC9E2BCECE33803EAC14DE7E8E988FC9C8DC8B|acpK]-[gene|85D6E439529E8B3F73244A0B004B394B4FE65509|pksF]-[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]-[gene|A4CF68F602273CFDFD55EFD2C1164F0E2E8F6972|pksH]-[gene|F437A90A97CF8ED4BA1E5CCA8A33D9E93612902B|pksI]-[gene|13EB6B903088C11D985DDB7CEC864797058FE038|pksJ]-[gene|BABBC5848F9BBF9E9318252F5AD7D1C4F55FCEAB|pksL]-[gene|40779D3A6E4F72EEFD702BF9FA15B57951783D12|pksM]-[gene|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|pksN]-[gene|85213235F7D3D8E711963E53F314ED758B5FA94B|pksR]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|24187085], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: activation, [Pubmed|24187085], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • expressed during the transition from growth to stationary phase ([protein|search|AbrB], [protein|search|CodY]) [Pubmed|24187085]

  additional information

  • this is a very large operon comprising about 75 kb

• genes: [gene|647B1D7E496E81E7FC7FD2BDCACC2486852BAC19|pksB]-[gene|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC]-[gene|F3E9CA9330394FD829D59B0C2D4D1EEDD2C22D3B|pksD]-[gene|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE]-[gene|6BEC9E2BCECE33803EAC14DE7E8E988FC9C8DC8B|acpK]-[gene|85D6E439529E8B3F73244A0B004B394B4FE65509|pksF]-[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]-[gene|A4CF68F602273CFDFD55EFD2C1164F0E2E8F6972|pksH]-[gene|F437A90A97CF8ED4BA1E5CCA8A33D9E93612902B|pksI]-[gene|13EB6B903088C11D985DDB7CEC864797058FE038|pksJ]-[gene|BABBC5848F9BBF9E9318252F5AD7D1C4F55FCEAB|pksL]-[gene|40779D3A6E4F72EEFD702BF9FA15B57951783D12|pksM]-[gene|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|pksN]-[gene|85213235F7D3D8E711963E53F314ED758B5FA94B|pksR]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-A017 (pksG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/17 NBRP B. subtilis, Japan]
• BKE17150 (Δ[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCTTCTATTCCGGCTGAAA, downstream forward: _UP4_TCTGAGTTTCATCGGAAGTA
• BKK17150 (Δ[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCTTCTATTCCGGCTGAAA, downstream forward: _UP4_TCTGAGTTTCATCGGAAGTA

References

• 23840410,22383849,24187085,27766092