xylB [Mon Aug 31 2015 13:54:31 GMT+0200 (CEST)]

xylulokinase

locus: BSU17610

pI: 6.46

mw: 55.00 kDa

function: utilization of xylan and xylose

product: xylulokinase

essential: no

ec: 2.7.1.17

synonyms: yncA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.5|Utilization of xylan/ xylose]

syns: yncA

[SW|Categories] containing this gene/protein

• [SW|utilization of specific carbon sources]

This gene is a member of the following [SW|regulons]

• [SW|CcpA regulon], [SW|XylR regulon]

The protein

Catalyzed reaction/ biological activity

• ATP + D-xylulose = ADP + D-xylulose 5-phosphate (according to Swiss-Prot)

Protein family

• FGGY kinase family (according to Swiss-Prot)

Structure

• [PDB|2NLX] (from ''E. coli'', 35% identity, 52% similarity) [Pubmed|17123542]

Expression and Regulation

Operon

• ''[protein|search|xylA]-[protein|search|xylB]'' [Pubmed|1921970]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|2454911]

Regulation

• carbon catabolite repression ([protein|search|CcpA]) [Pubmed|8132469]
• induction by xylose ([protein|search|XylR]) [Pubmed|2454911]

Regulatory mechanism

• [protein|search|CcpA]: transcription repression [Pubmed|8132469]
• [protein|search|XylR]: transcription repression [Pubmed|2454911]

Biological materials

Mutant

• GP1151 (del aphA3) available in [SW|Stülke] lab
• 1A719 (no resistance), [Pubmed|1719948], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A719&Search=1A719 BGSC]

Labs working on this gene/protein

• [SW|Wolfgang Hillen], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]

References

• 17123542,1921970,2454911,8132469