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rplV
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rplV

ribosomal protein L22, important for folding and stabilization of the conformation of 23S rRNA
Locus
BSU_01210
Isoelectric point
11.23
Molecular weight
12.32 kDa
Protein length
113 aa Sequence Blast
Gene length
342 bp Sequence Blast
Function
translation
Product
ribosomal protein L22 (BL17)
Essential
yes
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → Ribosomal proteins
  • Groups of genes → Essential genes
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

    • Gene

    Coordinates
    138,497 → 138,838

    Phenotypes of a mutant

  • essential PubMed
  • loop duplication results in increased growth rate PubMed
  • loop duplication results in reduced hyperpolarization of cells PubMed
  • loop duplication results in increased expression of mgtE PubMed

    • The protein

    Protein family

  • universal ribosomal protein uL22 family (single member, according to UniProt)

    • Structure

  • 3J9W (the ribosome) PubMed

    • Localization

  • ribosome

    • Expression and Regulation

    Operons

    Genes
    rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-ybzG-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in SigA regulon

    • Regulatory mechanism

  • stringent response: negative regulation, in stringent response

    • Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed
  • likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed

    • Additional information

  • term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed
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    Biological materials

    Mutant

  • BKE01210 (ΔrplV::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTAAAAGCCTCCTCTCT, downstream forward: _UP4_TCAGAAAAGAAGGAGGGATA

    • References

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