mcpA

membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, acts also as pH sensor, responds to decreasing pH as an attractant signal

Locus: BSU_31240

Isoelectric point: 4.99

Molecular weight: 72.22 kDa

Protein length: 661 aa

Gene length: 1986 bp

Function: control of chemotaxis, sensing of acidic enviromnents

Product: methyl-accepting chemotaxis protein

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Signal transduction in motility and chemotaxis → Membrane-bound chemoreceptors
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,206,169 → 3,208,154

The protein

Paralogous protein(s)

• McpB, TlpB, TlpA, McpC

Domains

• Cache domain (aa 152-228) (according to UniProt)
• HAMP domain (aa 303-355) (according to UniProt)
• Methyl-accepting transducer domain (aa 374-610) (according to UniProt)

Modification

• deamination of Gln-586, Gln-593, and Gln-594 (by CheD) PubMed

Structure

• 6S1K (from E. coli, corresponds to aa 291 ... 555, 31% identity) PubMed

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: tlpA-mcpA
  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon

  Additional information

  • in minimal medium, McpA is present with 15,900 +/- 3,000 molecules per cell PubMed

Biological materials

Mutant

• BKE31240 (ΔmcpA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCATTCATTTCTCCTTTT, downstream forward: _UP4_TAATAAGCCTTAACACCCAA
• BKK31240 (ΔmcpA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCATTCATTTCTCCTTTT, downstream forward: _UP4_TAATAAGCCTTAACACCCAA

References

Reviews

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Original publications

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