SubtiBank SubtiBank
glmM
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

glmM

phosphoglucosamine mutase, required for cell wall synthesis
locus
BSU01770
pI
4.68
mw
48.27 kDa
protein length
448 aa Sequence Blast
gene length
1344 bp Sequence Blast
function
cell wall synthesis
product
phosphoglucosamine mutase, required for cell wall synthesis
essential
yes
ec
5.4.2.10
synonyms
ybbT

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • Gene

    Coordinates
    198,497 → 199,843

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate (according to Swiss-Prot)
  • Protein family

  • phosphohexose mutase family (according to Swiss-Prot)
  • Modification

  • phosphorylation on Ser-100 [Pubmed|17218307,17726680,20097688], this is an autophosphorylation [Pubmed|20097688]
  • Structure

  • [PDB|3PDK] (from ''B. anthracis'') [Pubmed|21685296]
  • Expression and Regulation

    Operons

    genes
    [gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]-[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]-[gene|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM]-[gene|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS]
    description
    [Pubmed|23192352]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22211522], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Expression vector

  • pGP400: (expression in ''B. subtilis'' in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP1401: (expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP1402: (cloning vector for glmM (GlmM S100A) in ''E. coli'', in [SW|pBlueskript KS]), available in [SW|Jörg Stülke]'s lab
  • pGP1403: (expression of GlmM (S100A) in ''B. subtilis'' in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP1405: (expression, purification of GlmM (S100A) in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • lacZ fusion

  • GP1340 (cat) based on [protein|search|pAC6], available in [SW|Jörg Stülke]'s lab
  • GFP fusion

  • GP1382 ''glmM-gfp ermC'' (based on [SW|pGP1080]), available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP1380 ''lacA::cdaR-Strep aphA3 glmM-3xFLAG ermC'' (based on [SW|pGP1460] and [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab
  • Antibody

  • available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 28683921,18266853
  • Original publications

  • 17726680,17218307,8550580,10231382,10671448,20097688,20525796,21685296,23192352,22211522,26240071,26585449,28516784