SubtiBank SubtiBank
hbs
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

hbs

non-specific DNA-binding protein Hbsu
locus
BSU22790
pI
9.50
mw
9.75 kDa
protein length
gene length
276 bp Sequence Blast
function
DNA packaging, function of the signal recognition complex
product
non-specific DNA-binding protein Hbsu
essential
yes
synonyms
dbpA,hupA

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
  • Gene

    Coordinates
    2,385,543 → 2,385,821

    Phenotypes of a mutant

  • essential [Pubmed|12682299,1382620]
  • The protein

    Catalyzed reaction/ biological activity

  • [protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|Hbs]-mediated untwisting of the DNA stimulates [protein|C352560155F5B2E951B5059794D7B319C1A3AF2C|Nth] endonuclease activity at AP sites [Pubmed|21954439]
  • Protein family

  • DbpA subfamily (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|YonN]
  • Modification

  • phosphorylated on Arg-61 [Pubmed|22517742]
  • phosphorylation on Thr-4 [Pubmed|17218307]
  • Structure

  • [PDB|1HUU] (Geobacillus stearothermophilus)
  • [SW|Localization]

  • associated with the nucleoid in vegetative cells and forespores [Pubmed|9068655,10715001]
  • membrane associated [Pubmed|18763711]
  • Expression and Regulation

    Operons

    genes
    [gene|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|hbs]
    description
    [Pubmed|1902464]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1902464], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|1902464], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]
  • regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]
  • regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]) [Pubmed|16267290]
  • additional information

  • mRNA degradation is initiated by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|28977557]
  • Hbs is present with about 50,000 copies per genome in vegetative cells and spores, it is one of the most abundant proteins in B. subtilis [PubMed|10715001]
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    Biological materials

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab
  • Labs working on this gene/protein

  • [SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
  • [SW|Mohamed Marahiel], University of Marburg, Germany [http://www.uni-marburg.de/fb15/ag-marahiel/Prof.%20Dr.%20Mohamed%20A.%20Marahiel Homepage]
  • References

    Reviews

  • 11931565
  • Original publications

  • 1382620,1902464,10715001,9068655,9894920,16267290,19458035,19210617,18763711,17218307,21954439,22517742,23033921,10231583,8748031,7852372,9106208,28977557