yhjB

similar to metabolite permease

locus: BSU_10450

pI: 9.63

mw: 52.60 kDa

protein length: 489 aa

gene length: 1470 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.7|Sodium-solute symporter]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.12|Other transporters]

Gene

Coordinates: 1,119,162 → 1,120,631

The protein

Protein family

• [SW|sodium:solute symporter (SSF) (TC 2.A.21) family] (according to UniProt)

Paralogous protein(s)

• [protein|7E86EB6FF86F2C4AE3FD11DB8CE8E71696D94B1B|YodF]

Expression and Regulation

Operons

• genes: [gene|F093B021240236AA938C6336AA0B8DC37839F569|yhjC]-[gene|EFA18ACD034D48868CD97470F81F23976DA300DF|yhjB]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [pubmed|12618455] [pubmed|18083814], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

  regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]

Biological materials

Mutant

• GP2395 Δ[gene|EFA18ACD034D48868CD97470F81F23976DA300DF|yhjB]::tet available in [SW|Jörg Stülke]'s lab [pubmed|32743959]
• MGNA-B283 (yhjB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1282 NBRP B. subtilis, Japan]
• BKE10450 (Δ[gene|EFA18ACD034D48868CD97470F81F23976DA300DF|yhjB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTCATTCTGCTTCCTCCTT, downstream forward: _UP4_TAAGCATAAAAAAAGCAATC
• BKK10450 (Δ[gene|EFA18ACD034D48868CD97470F81F23976DA300DF|yhjB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTCATTCTGCTTCCTCCTT, downstream forward: _UP4_TAAGCATAAAAAAAGCAATC

References

• 11948146,22383849