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yfmT
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yfmT

vanillin dehydrogenase
locus
BSU_07350
pI
5.08
mw
53.15 kDa
protein length
485 aa Sequence Blast
gene length
1458 bp Sequence Blast
function
unknown
product
vanillin dehydrogenase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    807,091 → 808,548

    The protein

    Catalyzed reaction/ biological activity

  • oxidation of vanillin to vanillic acid [Pubmed|26658822]
  • benzaldehyde + H2O + NAD+ --> benzoate + 2 H+ + NADH (according to UniProt)
  • 4-hydroxy-3-methoxybenzaldehyde + H2O + NAD+ --> 4-hydroxy-3-methoxybenzoate + 2 H+ + NADH (according to UniProt)

    • Protein family

  • [SW|aldehyde dehydrogenase family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|F3341F205CB939498109D2A54DE842065C488DD5|AldX], [protein|F9CF067A2A8E3B2BACC6A51A0E87E84640349980|AldY], [protein|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|GbsA], [protein|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|YcbD], [protein|67A72A0EABCD807C25D8EAC61C251142B45C174E|DhaS], [protein|762718A15E5256261D79DF60F9106AF0CE2D60C6|IolA], [protein|99F1FAF28FB4817D94E84BD5288FA33124558933|YwdH], [protein|A0BEB92D54799956A4ADE106A1388E5710141069|GabD], [protein|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|PutC], [protein|69838717DC6BB27864D88C282BF5BC7CC558BFD7|RocA]

    • Structure

  • [PDB|4DNG] (from ''Bacillus Subtilis'', 47% identity)
  • [PDB|3RHH] (from from ''Bacillus halodurans'' C-125 complexed with NADP, 32% identity, 67% similarity)

    • Expression and Regulation

    Operons

    genes
    [gene|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|yfmT]-[gene|63BCB42FC501B57ABC9FF252AC5D04BB729EA6B9|yfmS]
    description
    [Pubmed|9141694,15033535]

    sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|15033535], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

    • additional information

  • in minimal medium, YfmS is present with 4,200 +/- 800 molecules per cell [PubMed|21515776]
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    Biological materials

    Mutant

  • MGNA-C232 (yfmT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2230 NBRP B. subtilis, Japan]
  • BKE07350 (Δ[gene|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|yfmT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTCAGCATCTCCTTTA, downstream forward: _UP4_TTCCCTTATTAATGAAAAGG
  • BKK07350 (Δ[gene|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|yfmT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTCAGCATCTCCTTTA, downstream forward: _UP4_TTCCCTTATTAATGAAAAGG

    • labs

  • [SW|Josef Altenbuchner]

    • References

  • 9141694,15033535,26658822