ykuP

flavodoxin, binds FMN, replaces ferredoxin under conditions of iron limitation

locus: BSU_14170

pI: 4.10

mw: 20.11 kDa

protein length: 151 aa

gene length: 456 bp

function: electron transfer

product: flavodoxin

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.3|Electron transport/ other]

Gene

Coordinates: 1,488,413 → 1,488,868

The protein

Protein family

• flavodoxin family (with [protein|0932DDC285635C02067A3302CEDA302FE4269179|YkuN], according to UniProt)

Paralogous protein(s)

• [protein|0932DDC285635C02067A3302CEDA302FE4269179|YkuN]

[SW|Domains]

• [SW|Flavodoxin-like domain] (aa 4-144) (according to UniProt)

[SW|Cofactors]

• FMN

Structure

• [PDB|6FSG] (from B. cereus, 46% identity) [pubmed|29722453]

Expression and Regulation

Operons

• genes: [gene|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]-[gene|CEC1B5DF1E548597FE333895ECA476AB9DFFFDD7|ykuO]-[gene|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP]
  description: [Pubmed|12354229]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12354229], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI]: sigma factor, [pubmed|29914988], in [regulon|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI regulon]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|24214949], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • [protein|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|Kre]: repression, in [regulon|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|Kre regulon]
  • [protein|EC6697D5D945B7E5083AFED9218748763C443278|NsrR]: repression, [Pubmed|24214949], in [regulon|EC6697D5D945B7E5083AFED9218748763C443278|NsrR regulon]
  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

  regulation

  • expressed under anaerobic conditions ([protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]) [Pubmed|24214949]
  • induced by iron starvation (second wave) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [pubmed|29133393,12354229]
  • expression of the operon is strongly induced at the beginning of [SW|biofilm formation] [pubmed|31113899]

  additional information

  • the ''[protein|0932DDC285635C02067A3302CEDA302FE4269179|YkuN]-[protein|CEC1B5DF1E548597FE333895ECA476AB9DFFFDD7|YkuO]-[protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|YkuP]'' operon is strongly unregulated in a ''[SW|kre]'' mutant [Pubmed|26110430]

Biological materials

Mutant

• MGNA-B339 (ykuP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1338 NBRP B. subtilis, Japan]
• BKE14170 (Δ[gene|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCCTTGTTCCTCCTCT, downstream forward: _UP4_TGATCACTCACTGGGAACTG
• BKK14170 (Δ[gene|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCCTTGTTCCTCCTCT, downstream forward: _UP4_TGATCACTCACTGGGAACTG

References

• 15449930,17127770,12354229,12107147,20186410,24214949,21665975,29133393,29914988,29722453