SubtiBank SubtiBank
glpK

glpK

glycerol kinase
locus
BSU09290
pI
4.99
mw
54.91 kDa
protein length
496 aa Sequence Blast
gene length
1488 bp Sequence Blast
function
glycerol utilization
product
glycerol kinase
essential
no
ec
2.7.1.30
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.3|Utilization of glycerol/ glycerol-3-phosphate]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]
  • Gene

    Coordinates
    1,003,344 → 1,004,834

    The protein

    Catalyzed reaction/ biological activity

  • ATP + glycerol = ADP + sn-glycerol 3-phosphate (according to Swiss-Prot)
  • Protein family

  • FGGY kinase family (according to Swiss-Prot)
  • Modification

  • phosphorylation (His230) (according to Swiss-Prot)
  • Structure

  • [PDB|3H46] (from ''Enterococcus casseliflavus'', complex with glycerol) [Pubmed|19102629]
  • Expression and Regulation

    Operons

    genes
    [gene|B12432503801FA766D6FC90359A22A53A8E92457|glpF]-[gene|ECA8EBD553936CDD371B947D48B3C7D049B755BD|glpK]
    description
    [Pubmed|2127799]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2127799], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|38767691AE7E09F46B9E97A60BF5358C1876EDF8|GlpP]: antitermination, /antitermination via [protein|38767691AE7E09F46B9E97A60BF5358C1876EDF8|GlpP]-dependent [SW|RNA switch], in [regulon|38767691AE7E09F46B9E97A60BF5358C1876EDF8|GlpP regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: repression, [pubmed|28439033], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • regulation

  • induction by glycerol ([protein|38767691AE7E09F46B9E97A60BF5358C1876EDF8|GlpP]) [Pubmed|8436953]
  • repressed under anaerobic conditions ([protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]) [pubmed|28439033,10913079]
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    Biological materials

    Mutant

  • BKE09290 (''glpK''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
  • GP1865 (''glpK''::''ermC'') (available in [SW|Jörg Stülke]'s lab)
  • Labs working on this gene/protein

  • [SW|Josef Deutscher], Paris-Grignon, France
  • References

  • 1335945,9162046,11929549,7773413,19102629,20817675,28439033,10913079,28458661