murF [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine synthetase

locus: BSU04570

pI: 5.00

mw: 49.00 kDa

function: peptidoglycan precursor biosynthesis

product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-
2,6- diaminopimeloyl-D-alanyl-D-alanine synthetase

essential: yes

ec: 6.3.2.10

synonyms: ydbQ

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|cell wall synthesis], [SW|biosynthesis of cell wall components], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|essential genes]

This gene is a member of the following [SW|regulons]

• [SW|SigM regulon]

Gene

Coordinates on the chromosome (coding sequence): 509,384 -> 510,757

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine D-alanyl-D-alanine = ADP phosphate UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine (according to Swiss-Prot)

Protein family

• murCDEF family (according to Swiss-Prot)

Structure

• [PDB|1GG4] (from ''Escherichia coli'', 32% identity, 50% similarity) [Pubmed|11090285]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)
• forms banded patterns along the length of the cell, this pattern formation depends on [SW|MreB] [Pubmed|20233306]

Expression and Regulation

Operon

• ''[SW|ddl]-[SW|murF]'' [Pubmed|22383849]

[SW|Sigma factor]

• [protein|search|SigM] [Pubmed|18179421]

Regulation

• expression is reduced in a [protein|search|SigV] mutant [Pubmed|21926231]

References

• 18063720,18179421,20233306