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rpoB [2020-10-21 12:28:25]
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rpoB [2020-10-21 12:28:25]

[SW|RNA polymerase] beta subunit
locus
BSU_01070
pI
4.73
mw
133.38 kDa
protein length
1193 aa Sequence Blast
gene length
3582 bp Sequence Blast
function
[SW|transcription]
product
[SW|RNA polymerase] beta subunit
essential
yes
ec
2.7.7.6
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.1|RNA polymerase]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • Gene

    Coordinates
    121,919 → 125,500

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • Nucleoside triphosphate + RNA(n) --> diphosphate + RNA(n + 1) (according to UniProt)

    • Protein family

  • [SW|RNA polymerase] beta chain family (single member, according to UniProt)

    • Modification

  • phosphorylated on (Arg-312 OR Arg-313), Arg-539, (Arg-693 OR Arg-694), Arg-827, and Arg-1106 [Pubmed|22517742]

    • Structure

  • [PDB|3IYD] ([SW|RNA polymerase] from ''E. coli'', 59% identity) [Pubmed|19903881]
  • [PDB|3MLQ] (RNA polymerase interacting domain of ''Thermus thermophilus'' [protein|AD1F39823ABCE2A222259B879513752942C59590|Mfd] with the ''Thermus aquaticus'' [protein|EC32328548BFB59D9026ACE351C946DBBC0932BC|RpoB] beta1 domain) [Pubmed|20702425]

    • [SW|Localization]

  • cytoplasm

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

    • Biological materials

    lacZ fusion

  • pGP2683 (cat) based on [SW|pAC5], available in [SW|Jörg Stülke]'s lab
  • pGP2682 (aphA3) based on [SW|pAC7], available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

    • FLAG-tag construct

  • GP2043 ''rpoB-3xFLAG ermC'' (based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 22210308,24763425,28657884

    • Original publications

  • 19680289,17644585,24491578,7657605,12486038,10675340,7592585,20525796,17981983,18763711,19191541,21512256,21821766,22517742,24572018,22484477,20702425,20817769,26293966,26538577,19903881,26098117,28539362,29794222