SubtiBank SubtiBank
rpoB [Sun Nov 27 2016 17:54:02 GMT+0100 (CET)]
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rpoB [Sun Nov 27 2016 17:54:02 GMT+0100 (CET)]

RNA polymerase beta subunit
locus
BSU01070
pI
4.00
mw
133.00 kDa
function
transcription
product
RNA polymerase beta subunit
essential
yes
ec
2.7.7.6
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.1|RNA polymerase]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [SW|Categories] containing this gene/protein

  • [SW|transcription], [SW|essential genes], [SW|membrane proteins], [SW|phosphoproteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|stringent response]
  • Gene

    Coordinates on the chromosome (coding sequence)
    121,919 -> 125,500

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Nucleoside triphosphate RNA(n) = diphosphate RNA(n 1) (according to Swiss-Prot)
  • Protein family

  • [SW|RNA polymerase] beta chain family (according to Swiss-Prot)
  • Modification

  • phosphorylated on (Arg-312 OR Arg-313), Arg-539, (Arg-693 OR Arg-694), Arg-827, and Arg-1106 [Pubmed|22517742]
  • Structure

  • [PDB|3IYD] ([SW|RNA polymerase] from ''E. coli'', 59% identity) [Pubmed|19903881]
  • [PDB|3MLQ] (RNA polymerase interacting domain of ''Thermus thermophilus'' [protein|search|Mfd] with the ''Thermus aquaticus'' [protein|search|RpoB] beta1 domain) [Pubmed|20702425]
  • [SW|Localization]

  • membrane associated [Pubmed|18763711]
  • [SW|Interactions]

  • [protein|search|RpoA]-[protein|search|RpoB]-[protein|search|RpoC] [Pubmed|10499798], [protein|search|NusA]-[protein|search|RpoB] [Pubmed|19680289]
  • [protein|search|SigA]-([protein|search|RpoB]-[protein|search|RpoC]) [Pubmed|19680289,19735077], [protein|search|SigB]-([protein|search|RpoB]-[protein|search|RpoC])
  • [protein|search|SigD]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|SigE]-([protein|search|RpoB]-[protein|search|RpoC])
  • [protein|search|SigF]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|SigG]-([protein|search|RpoB]-[protein|search|RpoC])
  • [protein|search|SigH]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|SigI]-([protein|search|RpoB]-[protein|search|RpoC])
  • [protein|search|SigK]-([protein|search|RpoB]-[protein|search|RpoC])
  • [protein|search|SigL]-([protein|search|RpoB]-[protein|search|RpoC]-[protein|search|RpoA]) [Pubmed|26293966]
  • [protein|search|SigM]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|SigV]-([protein|search|RpoB]-[protein|search|RpoC])
  • [protein|search|SigW]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|SigX]-([protein|search|RpoB]-[protein|search|RpoC])
  • [protein|search|SigY]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|SigZ]-([protein|search|RpoB]-[protein|search|RpoC])
  • [protein|search|Xpf]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|YlaC]-([protein|search|RpoB]-[protein|search|RpoC])
  • [protein|search|YvrI]-[protein|search|RpoB] [Pubmed|19940246],
  • [protein|search|Mfd]-[protein|search|RpoB] [Pubmed|20702425]
  • [protein|search|RpoB]-[protein|search|RpoE] (demonstrated for ''S. aureus'') [Pubmed|24491578]
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
  • Expression and Regulation

    Operon

  • ''[protein|search|rpoB]-[protein|search|rpoC]'' [Pubmed|22383849]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|7657605]
  • Regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • Biological materials

    lacZ fusion

  • pGP2683 (cat) based on [protein|search|pAC5], available in [SW|Jörg Stülke]'s lab
  • pGP2682 (aphA3) based on [protein|search|pAC7], available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab
  • FLAG-tag construct

  • GP2043 ''rpoB-3xFLAG ermC'' (based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 22210308,24763425
  • Original publications

  • 19680289,17644585,24491578,7657605,12486038,10675340,7592585,20525796,17981983,18763711,19191541,21512256,21821766,22517742,24572018,22484477,20702425,20817769,26293966,26538577,19903881