SubtiBank SubtiBank
rpoB [2017-07-13 10:40:05]
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rpoB [2017-07-13 10:40:05]

RNA polymerase beta subunit
locus
BSU01070
pI
4.73
mw
133.38 kDa
protein length
1193 aa Sequence Blast
gene length
3579 bp Sequence Blast
function
transcription
product
RNA polymerase beta subunit
essential
yes
ec
2.7.7.6
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.1|RNA polymerase]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • Gene

    Coordinates
    121,919 → 125,500

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Nucleoside triphosphate RNA(n) = diphosphate RNA(n 1) (according to Swiss-Prot)
  • Protein family

  • [SW|RNA polymerase] beta chain family (according to Swiss-Prot)
  • Modification

  • phosphorylated on (Arg-312 OR Arg-313), Arg-539, (Arg-693 OR Arg-694), Arg-827, and Arg-1106 [Pubmed|22517742]
  • Structure

  • [PDB|3IYD] ([SW|RNA polymerase] from ''E. coli'', 59% identity) [Pubmed|19903881]
  • [PDB|3MLQ] (RNA polymerase interacting domain of ''Thermus thermophilus'' [protein|AD1F39823ABCE2A222259B879513752942C59590|Mfd] with the ''Thermus aquaticus'' [protein|EC32328548BFB59D9026ACE351C946DBBC0932BC|RpoB] beta1 domain) [Pubmed|20702425]
  • [SW|Localization]

  • membrane associated [Pubmed|18763711]
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
  • Expression and Regulation

    Operons

    genes
    [gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB]-[gene|BC8B37355E6FADBA5F4636C3879E99003B0F3E84|rpoC]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7657605], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • the leader mRNA is processed upstream of [gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
  • view in new tab

    Biological materials

    lacZ fusion

  • pGP2683 (cat) based on [protein|search|pAC5], available in [SW|Jörg Stülke]'s lab
  • pGP2682 (aphA3) based on [protein|search|pAC7], available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab
  • FLAG-tag construct

  • GP2043 ''rpoB-3xFLAG ermC'' (based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 22210308,24763425
  • Original publications

  • 19680289,17644585,24491578,7657605,12486038,10675340,7592585,20525796,17981983,18763711,19191541,21512256,21821766,22517742,24572018,22484477,20702425,20817769,26293966,26538577,19903881,26098117,28539362