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pbpB [Mon Nov 02 2015 19:08:20 GMT+0100 (CET)]
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pbpB [Mon Nov 02 2015 19:08:20 GMT+0100 (CET)]

class B penicillin-binding protein 2B, Pbp2B
locus
BSU15160
pI
9.00
mw
79.00 kDa
function
septation, cell division
product
penicillin-binding protein 2B
essential
yes
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|cell wall synthesis], [SW|cell division], [SW|essential genes], [SW|membrane proteins], [SW|penicillin-binding proteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,581,947 -> 1,584,097

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Protein family

  • transpeptidase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [SW|SpoVD]

    • [SW|Domains]

  • contains two C-terminal [SW|PASTA domain]s (aa 598-657, 658-714)

    • Structure

  • [PDB|1RP5] (PBP 2x from ''Streptococcus pneumoniae'', 32% identity) [Pubmed|14734544]

    • [SW|Localization]

  • cell membrane [Pubmed|23869552,20870765]
  • division sites [Pubmed|23895585]
  • extracellular (signal peptide) [Pubmed|18957862]
  • localizes to the sporulation septum during sporulation [Pubmed|15758244]

    • [SW|Interactions]

  • folding requires [protein|search|PrsA] [Pubmed|20487272]
  • [protein|search|PbpB]-[SW|DivIB]-([protein|search|FtsL]-[SW|DivIC])2 [Pubmed|20870765], as part of the [SW|divisome]
  • [protein|search|WalK]-[protein|search|PbpB] [Pubmed|21219466]

    • Expression and Regulation

    Operon

  • ''[protein|search|mraZ]-[protein|search|mraW]-[protein|search|ftsL]-[protein|search|pbpB]'' [Pubmed|8636036,8244929]

    • Regulation

  • constitutively expressed [Pubmed|15758244]

    • Biological materials

    two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jeff Errington] lab

    • FLAG-tag construct

  • GP2003 ''pbpB-3xFLAG spec'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

    • References

  • 8636036,14731276,8244929,20487272,10652091,19429628,18957862,21219466,20870765,14734544,23895585,24655324