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sacY [Tue Jul 05 2016 18:05:17 GMT+0200 (CEST)]
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sacY [Tue Jul 05 2016 18:05:17 GMT+0200 (CEST)]

transcriptional antiterminator for sacB and sacX-sacY (acts at high sucrose concentrations)
locus
BSU38420
pI
5.00
mw
32.00 kDa
function
regulation of sucrose utilization
product
transcriptional antiterminator
essential
no
synonyms
ipa-13r, sacS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.19|Utilization of sucrose]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.3|PRD-type regulators]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.4|RNA binding regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • [SW|Categories] containing this gene/protein

  • [SW|utilization of specific carbon sources], [SW|transcription factors and their control], [SW|RNA binding regulators], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|SacY regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,943,667 -> 3,944,509

    The protein

    Catalyzed reaction/ biological activity

  • binding to the mRNA of'' [protein|search|sacB]'' and the ''[protein|search|sacX]-[protein|search|sacY]'' operon, causes transcription antitermination (in presence of sucrose)

    • Protein family

  • [PRD-containing transcription factors|search|transcriptional antiterminator] bglG family (according to Swiss-Prot) BglG family of antiterminators

    • Paralogous protein(s)

  • [protein|search|LicT], [protein|search|GlcT], [protein|search|SacT]

    • Structure

  • [PDB|1AUU] (RNA-binding domain)

    • [SW|Interactions]

  • [protein|search|PtsH]-[protein|search|SacY]

    • Expression and Regulation

    Operon

  • ''[protein|search|sacX]-[protein|search|sacY]'' [Pubmed|2116367]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|1400159]

    • Regulation

  • induction by sucrose (at high concentration) [Pubmed|8535520]

    • Regulatory mechanism

  • induction by [protein|search|SacY]-dependent [SW|RNA switch] (transcriptional antitermination) [Pubmed|8535520]
  • [protein|search|DegU]-P: transcription activation [Pubmed|1400159]

    • Biological materials

    Mutant

  • GP425 (cat), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP316, available in [SW|Jörg Stülke]'s lab
  • for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [SW|pGP570]: pGP573, available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1226 (spc, based on [SW|pGP1870]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1222 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

    • References

  • 2105292,8702561,11580842,1279678,9305643,9305644,9202047,10610766,2116367,1400159,8535520,21278164,23303779,12079345