yqjM

NADPH-dependent flavin oxidoreductase

locus: BSU_23820

pI: 5.14

mw: 37.43 kDa

protein length: 338 aa

gene length: 1017 bp

function: reduction of double bonds of nonsaturated aldehydes

product: NADPH-dependent flavin oxidoreductase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 2,475,843 → 2,476,859

The protein

Catalyzed reaction/ biological activity

• A + H⁺ + NADPH --> AH₂ + NADP⁺ (according to UniProt)

Protein family

• NADH:flavin oxidoreductase/NADH oxidase family (with [protein|9DC6FD87D1AF7CD2F41738E04C4D6DCD6F6276E5|YqiG], according to UniProt)

Paralogous protein(s)

• [protein|9DC6FD87D1AF7CD2F41738E04C4D6DCD6F6276E5|YqiG]

[SW|Cofactors]

• FMN [pubmed|15890652]

Structure

• [PDB|1Z41] (oxidized form), [PDB|1Z48] (reduced form) [pubmed|15890652]

Expression and Regulation

Operons

• genes: [gene|EB17629B7598C62741072E1AF0AB715C02041B70|yqjM]
  description: [Pubmed|22383849]

  regulation

  • induced in the presence of H₂O₂ [Pubmed|12660247]

Biological materials

Mutant

• BKE23820 (Δ[gene|EB17629B7598C62741072E1AF0AB715C02041B70|yqjM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTTCGCCTCCCGTA, downstream forward: _UP4_TAATGCCCATAAGAGATATC
• BKK23820 (Δ[gene|EB17629B7598C62741072E1AF0AB715C02041B70|yqjM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTTCGCCTCCCGTA, downstream forward: _UP4_TAATGCCCATAAGAGATATC

References

Reviews

• 25940546

Original publications

• 12660247,15249051,15890652,26521678,30379645,30645192