GTPase, nucleotide-binding protein
function
required for the expression of late competence genes
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence][SW|Categories] containing this gene/protein
[SW|genetic competence]Gene
Coordinates on the chromosome (coding sequence)
3,571,501 -> 3,572,388
Phenotypes of a mutant
reduced [SW|genetic competence] [Pubmed|19074378]The protein
Catalyzed reaction/ biological activity
binds and hydrolyzes nucleotides, required for the expression of late competence genes (''[protein|search|comK], [protein|search|comS]'') [Pubmed|19074378]Protein family
UPF0042 family (according to Swiss-Prot)[SW|Localization]
can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium [Pubmed|21709426]Additional information
[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=retrieve&dopt=full_report&list_uids=947727&log$=genesensor5&logdbfrom=pubmed Information on the ''E. coli'' homolog]Expression and Regulation
Operon
''[protein|search|yvcI]-[protein|search|yvcJ]-[protein|search|yvcK]-[protein|search|yvcL]-[protein|search|crh]-[protein|search|yvcN]'' [Pubmed|9237995][SW|Sigma factor]
[protein|search|SigA] [Pubmed|16272399]Regulation
very weak stimuation of expression by citrate and succinate [Pubmed|16272399]Biological materials
Mutant
1A885 ( ''yvcJ''::''cat''), [Pubmed|19074378], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A885&Search=1A885 BGSC]Expression vector
pGP735 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] labtwo-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Görke] labLabs working on this gene/protein
[SW|Anne Galinier], University of Marseille, FranceReferences
Original publications
9237995,16272399,19074378,21709426 Publications on the ''E. coli'' homolog