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yvcJ [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
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yvcJ [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

GTPase, nucleotide-binding protein
locus
BSU34770
pI
6.70
mw
33.00 kDa
function
required for the expression of late competence genes
product
GTPase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]

    • [SW|Categories] containing this gene/protein

  • [SW|genetic competence]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,571,501 -> 3,572,388

    Phenotypes of a mutant

  • reduced [SW|genetic competence] [Pubmed|19074378]

    • The protein

    Catalyzed reaction/ biological activity

  • binds and hydrolyzes nucleotides, required for the expression of late competence genes (''[protein|search|comK], [protein|search|comS]'') [Pubmed|19074378]

    • Protein family

  • UPF0042 family (according to Swiss-Prot)

    • [SW|Localization]

  • can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium [Pubmed|21709426]

    • Additional information

  • [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=retrieve&dopt=full_report&list_uids=947727&log$=genesensor5&logdbfrom=pubmed Information on the ''E. coli'' homolog]

    • Expression and Regulation

    Operon

  • ''[protein|search|yvcI]-[protein|search|yvcJ]-[protein|search|yvcK]-[protein|search|yvcL]-[protein|search|crh]-[protein|search|yvcN]'' [Pubmed|9237995]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|16272399]

    • Regulation

  • very weak stimuation of expression by citrate and succinate [Pubmed|16272399]

    • Biological materials

    Mutant

  • 1A885 ( ''yvcJ''::''cat''), [Pubmed|19074378], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A885&Search=1A885 BGSC]

    • Expression vector

  • pGP735 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Görke] lab

    • Labs working on this gene/protein

  • [SW|Anne Galinier], University of Marseille, France

    • References

    Original publications

  • 9237995,16272399,19074378,21709426

    • Publications on the ''E. coli'' homolog

  • 17824929