deaD [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

DEAD-box RNA helicase, important for adaptation to low temperatures

locus: BSU39110

pI: 7.00

mw: 53.00 kDa

function: RNA helicase

product: DEAD-box RNA helicase

essential: no

synonyms: yxiN

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.3|DEAD-box RNA helicases]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.12|Translation/ other]

[SW|Categories] containing this gene/protein

• [SW|DEAD-box RNA helicases], [SW|translation]

Gene

Coordinates on the chromosome (coding sequence): 4,015,987 -> 4,017,426

The protein

Catalyzed reaction/ biological activity

• RNA helicase
• required for [SW|ribosome] assembly [Pubmed|23175651]

Protein family

• [DEAD-box RNA helicases|search|DEAD-box RNA helicase]

Paralogous protein(s)

• [protein|search|CshA], [protein|search|CshB], [protein|search|YfmL]

[SW|Domains]

• Helicase domain 1: AS 1-203
• Linker region: AS 204-212
• Helicase domain 2: AS 207-368
• RNA-binding domain: AS 404-479

Structure

• [PDB|2G0C] (RNA-binding domain, AA 404-479), [PDB|2HJV] (second domain, AA 207-368); [PDB|3MOJ] (RNA binding domain complexed with a fragment of 23S ribosomal RNA) [Pubmed|20673833]

[SW|Interactions]

• [protein|search|DeaD]-[protein|search|CshA] [Pubmed|20572937]
• [protein|search|DeaD]-[protein|search|CshB] [Pubmed|20572937]
• [protein|search|DeaD]-[SW|23S rRNA] [Pubmed|20673833]

Expression and Regulation

Operon

• ''[protein|search|yxiM]-[protein|search|deaD]'' [Pubmed|10746760]

Regulation

• expression declines in the stationary phase [Pubmed|23175651]

Biological materials

Mutant

• GP1052 (tet), available in the [SW|Stülke] lab

Expression vector

• for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from the native chromomsomal site: GP1065 (spc), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP1068 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Dagmar Klostermeier], Biozentrum Basel, Switzerland [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]

References

• 17142894,16118224,17229151,16611943,19839642,19474341,18184816,17951299,12460566,10481020,20572937,20673833,20691700,23175651,23625962,25907111,25123660