dxs [2019-07-02 10:17:30]

1-deoxyxylulose-5-phosphate synthase, first step in the MEP pathway of isoprenoid biosynthesis

locus: BSU24270

pI: 6.00

mw: 69.38 kDa

protein length: 633 aa

gene length: 1902 bp

function: biosynthesis of thiamine, MEP pathway of isoprenoid biosynthesis

product: 1-deoxyxylulose-5-phosphate synthase

essential: yes

ec: 2.2.1.7

synonyms: yqiE

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,523,713 → 2,525,614

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO₂ (according to Swiss-Prot)

Protein family

• DXPS subfamily (according to Swiss-Prot)

Structure

• [PDB|2O1S] (from ''E. coli'', 44% identity, 62% similarity) [Pubmed|17135236]

Expression and Regulation

Operons

• genes: [gene|EA9FCF84AE4FA80993566B62A9200D4B32AF5670|dxs]-[gene|923C4177500E9678A0B4855F8F481D4A36ECD863|yqxC]-[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

References

Reviews

• 19348578

Original publications

• 12882400,17135236,21296950,23840410,27856234