SubtiBank SubtiBank
dxs

dxs

1-deoxyxylulose-5-phosphate synthase, first step in the MEP pathway of isoprenoid biosynthesis
locus
BSU_24270
pI
6.00
mw
69.38 kDa
protein length
633 aa Sequence Blast
gene length
1902 bp Sequence Blast
function
biosynthesis of thiamine, MEP pathway of isoprenoid biosynthesis
product
1-deoxyxylulose-5-phosphate synthase
essential
yes
ec
2.2.1.7
synonyms
yqiE

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,523,713 → 2,525,614

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • D-glyceraldehyde 3-phosphate + H+ + pyruvate --> 1-deoxy-D-xylulose 5-phosphate + CO2 (according to UniProt)
  • Protein family

  • transketolase family (with [protein|000E237B00539E6850B6A50EE01BA02844B6ACBC|Tkt], according to UniProt)
  • Structure

  • [PDB|2O1S] (from ''E. coli'', 44% identity, 62% similarity) [Pubmed|17135236]
  • Expression and Regulation

    Operons

    genes
    [gene|EA9FCF84AE4FA80993566B62A9200D4B32AF5670|dxs]-[gene|923C4177500E9678A0B4855F8F481D4A36ECD863|yqxC]-[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]
  • regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
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    References

    Reviews

  • 19348578
  • Original publications

  • 12882400,17135236,21296950,23840410,27856234