ybdK

two-component sensor kinase

locus: BSU_02010

pI: 5.21

mw: 37.12 kDa

protein length: 320 aa

gene length: 963 bp

function: unknown

product: two-component sensor kinase

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 221,950 → 222,912

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|5F6D14A0B360832F9FD470668B006A628B109A56|YbdJ]
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

[SW|Domains]

• two transmembrane segments
• [SW|HAMP domain] (aa 67-120) (according to UniProt)
• [SW|Histidine kinase domain] (aa 135-320) (according to UniProt)

Modification

• autophosphorylation on a His residue

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|EA36C28A990EFFAEBFE279467A0B56B6B5F5255D|ybdK]
  description: [http://dbtbs.hgc.jp/COG/prom/ybdK.html DBTBS]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: positive regulation, in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

Biological materials

Mutant

• MGNA-B957 (ybdK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1956 NBRP B. subtilis, Japan]
• BKE02010 (Δ[gene|EA36C28A990EFFAEBFE279467A0B56B6B5F5255D|ybdK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGATATTTTGTCTTGA, downstream forward: _UP4_TGACCCCGCTGATGTTTTTC
• BKK02010 (Δ[gene|EA36C28A990EFFAEBFE279467A0B56B6B5F5255D|ybdK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGATATTTTGTCTTGA, downstream forward: _UP4_TGACCCCGCTGATGTTTTTC

References

• 10094672,11948146,20035725