SubtiBank SubtiBank
tsaC [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]
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tsaC [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

L-threonylcarbamoyl-AMP synthase, biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A) (together with TsaB-TsaD-TsaE)
locus
BSU36950
pI
5.00
mw
36.00 kDa
function
tRNA modification
product
L-threonylcarbamoyl-AMP synthase
essential
no
ec
2.7.7.87
synonyms
ipc-29d, ywlC

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [SW|Categories] containing this gene/protein

  • [SW|translation]
  • This gene is a member of the following [SW|regulons]

  • [SW|DnaA regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,792,969 -> 3,794,009

    Phenotypes of a mutant

  • restores viability of a ''[SW|smc]'' mutant on rich medium due to the induction of a [SW|stringent response] [Pubmed|26539825]
  • The protein

    Catalyzed reaction/ biological activity

  • conversion of L-threonine, bicarbonate/CO2 and ATP to give the intermediate L-threonylcarbamoyl-AMP (TC-AMP) and pyrophosphate as products [Pubmed|23072323]
  • biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A), a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro (shown for the corresponding ''E. coli'' protein YrdC) [Pubmed|19287007]
  • Protein family

  • SUA5 family (according to Swiss-Prot)
  • Structure

  • [PDB|1HRU] (the homolog from ''E. coli'') [Pubmed|19287007]
  • Expression and Regulation

    Operon

  • '' [protein|search|tsaC]'' [Pubmed|22383849]
  • Regulatory mechanism

  • [protein|search|DnaA]: transcription repression [Pubmed|17932079]
  • References

  • 17005971,17932079,19287007,23072323,22383849,26020636,26060251,26539825