L-threonylcarbamoyl-AMP synthase, biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A) (together with TsaB-TsaD-TsaE)
function
tRNA modification
product
L-threonylcarbamoyl-AMP synthase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes][category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation][SW|Categories] containing this gene/protein
[SW|translation]This gene is a member of the following [SW|regulons]
[SW|DnaA regulon]Gene
Coordinates on the chromosome (coding sequence)
3,792,969 -> 3,794,009
Phenotypes of a mutant
restores viability of a ''[SW|smc]'' mutant on rich medium due to the induction of a [SW|stringent response] [Pubmed|26539825]The protein
Catalyzed reaction/ biological activity
conversion of L-threonine, bicarbonate/CO2 and ATP to give the intermediate L-threonylcarbamoyl-AMP (TC-AMP) and pyrophosphate as products [Pubmed|23072323]biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A), a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro (shown for the corresponding ''E. coli'' protein YrdC) [Pubmed|19287007]Protein family
SUA5 family (according to Swiss-Prot)Structure
[PDB|1HRU] (the homolog from ''E. coli'') [Pubmed|19287007]Expression and Regulation
Operon
'' [protein|search|tsaC]'' [Pubmed|22383849]Regulatory mechanism
[protein|search|DnaA]: transcription repression [Pubmed|17932079]References
17005971,17932079,19287007,23072323,22383849,26020636,26060251,26539825 
