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21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.
ylaN
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other][category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.1|Acquisition of iron / Other][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]Gene
Coordinates
1,552,412 → 1,552,693
Phenotypes of a mutant
essential [Pubmed|12682299], the mutant is viable if iron(III) is added to the medium [Pubmed|27238023]The protein
Protein family
UPF0358 family (single member, according to UniProt)Structure
[PDB|2ODM] [Pubmed|17469204]Expression and Regulation
Operons
genes
[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|ylaN]
description
[Pubmed|22383849]
view in new tabBiological materials
Mutant
BKE14840 (Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|ylaN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAGTATCCCTCCGTTCC, downstream forward: _UP4_TAAATGATAAAACTCAAACTBKK14840 (Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|ylaN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAGTATCCCTCCGTTCC, downstream forward: _UP4_TAAATGATAAAACTCAAACTExpression vectors
IPTG inducible expression of Strep-''ylaN'' in ''E. coli'': pGP2579 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s labIPTG inducible expression of His-''ylaN'' in ''E. coli'': pGP2583 (in [SW|pWH844]), available in [SW|Jörg Stülke]'s labtwo-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s labAntibody
available in [SW|Jörg Stülke]'s labReferences
17005971,17469204,22383849,23420519,27238023,28189581,32949815