SubtiBank SubtiBank
tufA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

tufA

elongation factor Tu
locus
BSU01130
pI
4.72
mw
43.43 kDa
protein length
396 aa Sequence Blast
gene length
1188 bp Sequence Blast
function
translation
product
elongation factor Tu
essential
yes
ec
3.6.5.3
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]
  • Gene

    Coordinates
    132,882 → 134,072

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Protein family

  • EF-Tu/EF-1A subfamily (according to Swiss-Prot)
  • Modification

  • phosphorylated on Thr-384 by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC], dephosphorylated by [protein|84447F9A6644EA8A7593BB99B2B69D4377E670E2|PrpC] [Pubmed|19246764], [Pubmed|17726680]
  • Cys83 is S-bacillithiolated in B. subtilis and other Bacillus species [Pubmed|22938038]
  • phosphorylated on several Arg residues [Pubmed|24263382]
  • phosphorylated during [SW|sporulation] on Thr-63 by [protein|E86A96B832351FF513DD9853EAD8998CC44C9951|YabT], dephosphorylated by [protein|84447F9A6644EA8A7593BB99B2B69D4377E670E2|PrpC] [Pubmed|26056311]
  • phosphorylated on Tyr-270 [Pubmed|26381121]
  • Effectors of protein activity

  • phosphorylation by YabT results in inhibtion of GTP hydrolysis; as a result, EF-Tu remains stably bound
  • to the [SW|ribosome], stalling protein [SW|translation] [Pubmed|26056311]
  • Structure

  • [PDB|3U6B] (from ''E. coli '', 77% identity) [Pubmed|21999529]
  • [SW|Localization]

  • EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] [Pubmed|20133608]
  • membrane [Pubmed|18763711]
  • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]-[gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL]-[gene|8049CACE85B2627A08CB4D8A8587D52E2DB24C7F|rpsG]-[gene|297844645CCCF40CB40E84339997F6610883DF4E|fusA]-[gene|E771685D6D41D48E0C0AF77F15B4F380820FCDC2|tufA]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]
  • regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
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    Biological materials

    Expression vector

  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP839, available in [SW|Stülke] lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP847, available in [SW|Stülke] lab
  • References

  • 22938038,19246764,20133608,25707802,19246764,17726680,18763711,24263382,15378759,25676310,26056311,26381121,11918677,28516784