lutP

lactate permease

locus: BSU_34190

pI: 9.48

mw: 59.59 kDa

protein length: 563 aa

gene length: 1692 bp

function: lactate uptake

product: lactate permease

essential: no

synonyms: yvfH

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.4|Transporter for organic acids]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,510,780 → 3,512,471

Phenotypes of a mutant

• poor growth with lactate as single carbon source [Pubmed|19201793]

The protein

Catalyzed reaction/ biological activity

• lactate uptake [Pubmed|19201793]

Protein family

• lactate permease family (with [protein|84D391ED4E81BC17FC2A115985962445D7996DFA|LctP], according to UniProt)

Paralogous protein(s)

• [protein|84D391ED4E81BC17FC2A115985962445D7996DFA|LctP]

[SW|Localization]

• membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|E607B0D57398BCCF64CF3CE2110E632B0C3A109E|lutP]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|25031425], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]: activation, ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]) [Pubmed|16091051], in [regulon|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA regulon]
  • [protein|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|LutR]: repression, in [regulon|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|LutR regulon]

  regulation

  • expressed at high cell density ([protein|search|ComA]) [Pubmed|16091051]

Biological materials

Mutant

• MGNA-A489 (yvfH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/489 NBRP B. subtilis, Japan]
• BKE34190 (Δ[gene|E607B0D57398BCCF64CF3CE2110E632B0C3A109E|lutP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCAAACCCCTCCTTGA, downstream forward: _UP4_TAAAAAAATCCTCCCGTCTA
• BKK34190 (Δ[gene|E607B0D57398BCCF64CF3CE2110E632B0C3A109E|lutP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCAAACCCCTCCTTGA, downstream forward: _UP4_TAAAAAAATCCTCCCGTCTA

labs

• [SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

References

• 19201793,18763711,16091051,25031425,26582911