SubtiBank SubtiBank
accD [Wed Sep 23 2015 18:46:49 GMT+0200 (CEST)]
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accD [Wed Sep 23 2015 18:46:49 GMT+0200 (CEST)]

acetyl-CoA carboxylase (beta subunit)
locus
BSU29210
pI
5.00
mw
32.00 kDa
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (beta subunit))
essential
yes
ec
6.4.1.2
synonyms
yttI

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis of lipids], [SW|essential genes], [SW|phosphoproteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|Spo0A regulon]
  • Gene

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Modification

  • phosphorylated on Arg-205 [Pubmed|22517742]
  • Structure

  • [PDB|2F9I] (from ''Staphylococcus aureus''; equivalent to [protein|search|AccD]-[protein|search|AccA], 64% identity) [Pubmed|16460018]
  • [SW|Localization]

  • Membrane-proximal (Spotty) [Pubmed|16479537]
  • [SW|Interactions]

  • ([protein|search|AccA]-[protein|search|AccD])-[protein|search|AccB]-[protein|search|AccC] (acetyl-CoA carboxylase complex)
  • Expression and Regulation

    Operon

  • ''[protein|search|accD]-[protein|search|accA]'' [Pubmed|22383849]
  • Regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
  • Regulatory mechanism

  • [SW|Spo0A]: transcription activation [Pubmed|14651647]
  • Biological materials

    Expression vector

  • purification from ''B. subtilis'' with an C-terminal Strep-tag, for [SW|SPINE], (in [SW|pGP382]): pGP1724, available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP1497 (''mls'', based on [SW|pGP1370]), available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 15952903,12121720,15968460
  • Original Publications

  • 15066985,12663926,14651647,16479537,22517742,22383849,16460018,19965770