acetyl-CoA carboxylase (beta subunit)
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (beta subunit))
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue][SW|Categories] containing this gene/protein
[SW|biosynthesis of lipids], [SW|essential genes], [SW|phosphoproteins]This gene is a member of the following [SW|regulons]
[SW|Spo0A regulon]Gene
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Modification
phosphorylated on Arg-205 [Pubmed|22517742]Structure
[PDB|2F9I] (from ''Staphylococcus aureus''; equivalent to [protein|search|AccD]-[protein|search|AccA], 64% identity) [Pubmed|16460018][SW|Localization]
Membrane-proximal (Spotty) [Pubmed|16479537][SW|Interactions]
([protein|search|AccA]-[protein|search|AccD])-[protein|search|AccB]-[protein|search|AccC] (acetyl-CoA carboxylase complex)Expression and Regulation
Operon
''[protein|search|accD]-[protein|search|accA]'' [Pubmed|22383849]Regulation
expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]Regulatory mechanism
[SW|Spo0A]: transcription activation [Pubmed|14651647]Biological materials
Expression vector
purification from ''B. subtilis'' with an C-terminal Strep-tag, for [SW|SPINE], (in [SW|pGP382]): pGP1724, available in [SW|Jörg Stülke]'s labtwo-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s labFLAG-tag construct
GP1497 (''mls'', based on [SW|pGP1370]), available in [SW|Jörg Stülke]'s labReferences
Reviews
15952903,12121720,15968460 Original Publications
15066985,12663926,14651647,16479537,22517742,22383849,16460018,19965770 
