SubtiBank SubtiBank
accD
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

accD

acetyl-CoA carboxylase (beta subunit)
locus
BSU29210
pI
5.34
mw
28.73 kDa
protein length
262 aa Sequence Blast
gene length
786 bp Sequence Blast
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (beta subunit))
essential
yes
ec
6.4.1.2
synonyms
yttI

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • Gene

    Coordinates
    2,988,693 → 2,989,565

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Modification

  • phosphorylated on Arg-205 [Pubmed|22517742]
  • Structure

  • [PDB|2F9I] (from ''Staphylococcus aureus''; equivalent to [protein|E4997526CC534E38413030E90F7A135ECF65DF40|AccD]-[protein|22B3039DF4D106F121DBC765F49878C9B324FDB2|AccA], 64% identity) [Pubmed|16460018]
  • [SW|Localization]

  • Membrane-proximal (Spotty) [Pubmed|16479537]
  • Expression and Regulation

    Operons

    genes
    [gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD]-[gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
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    Biological materials

    Expression vector

  • purification from ''B. subtilis'' with an C-terminal Strep-tag, for [SW|SPINE], (in [SW|pGP382]): pGP1724, available in [SW|Jörg Stülke]'s lab
  • pGP1026, N-terminal Strep-tag, based on [SW|pGP172], purification from ''E. coli'', available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP1497 (''mls'', based on [SW|pGP1370]), available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 15952903,12121720,15968460
  • Original Publications

  • 15066985,12663926,14651647,16479537,22517742,22383849,16460018,19965770,28579978,28516784