bceB

primary bacitracin resistance determinant, [SW|ABC transporter] (permease), sensory component of the Bce regulatory & detoxification system, protects cell wall biosynthetic targets from inhibition by antimicrobial peptides

locus: BSU_30370

pI: 9.79

mw: 72.01 kDa

protein length: 646 aa

gene length: 1941 bp

function: protection of cell wall biosynthetic targets from inhibition by antimicrobial peptides

product: [SW|ABC transporter] for target protection of cell wall synthesis

essential: no

synonyms: ytsD

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.3|Export of peptides]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,109,397 → 3,111,337

Phenotypes of a mutant

• 85-fold increased sensitivity to bacitracin [Pubmed|26815905]

The protein

Catalyzed reaction/ biological activity

• binds bacitracin, K_D: 60 nM [Pubmed|25118291]
• inhibits [protein|08F24CA21DC54031F6158AC0C772F3B520E5A849|BceS] autophosphorylation activity in the absence of bacitracin [Pubmed|25118291]
• releaves intermediates of the lipid II cycle from the inhibitory interaction with antimicrobial peptides such as bacitracin [pubmed|31871088]

Protein family

• [SW|ABC-4 integral membrane protein family] (according to UniProt)

Effectors of protein activity

• the activity of the [protein|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|BceA]-[protein|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|BceB] [SW|ABC transporter] is inhibited by heptaprenyl pyrophosphate which accumulates in a ''[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]'' mutant [Pubmed|24806199]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]
  description: [Pubmed|12890034]

  regulatory mechanism

  • [protein|C239C155F5452C86BF6C78190BABBBB07A63DF87|BceR]: activation, [Pubmed|12890034], in [regulon|C239C155F5452C86BF6C78190BABBBB07A63DF87|BceR regulon]

  regulation

  • induced in the presence of bacitracin, plectasin, mersacidin and actagardine, already at very low levels of bacitracin ([protein|search|BceR]) [Pubmed|12890034,26815905]

Biological materials

Mutant

• GP3218 Δ([gene|C239C155F5452C86BF6C78190BABBBB07A63DF87|bceR]-[gene|08F24CA21DC54031F6158AC0C772F3B520E5A849|bceS]-[gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB])::''cat'', available in [SW|Jörg Stülke]'s lab
• MGNA-A168 (ytsD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/168 NBRP B. subtilis, Japan]
• BKE30370 (Δ[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAATGTTCATGCTGCAC, downstream forward: _UP4_TGAAACAGAAAAAGCTCCAG
• BKK30370 (Δ[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAATGTTCATGCTGCAC, downstream forward: _UP4_TGAAACAGAAAAAGCTCCAG

labs

• [SW|Susanne Gebhard], Bath, UK

References

Reviews

• 27344142,28152228,29404338

Original publications

• 18394148,17905982,12890034,14651641,10092453,14612242,21283517,20606066,21078927,23687272,25118291,24806199,26199330,26815905,31871088,32019833,32955745