SubtiBank SubtiBank
menD
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

menD

2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase
locus
BSU30820
pI
5.66
mw
63.92 kDa
protein length
580 aa Sequence Blast
gene length
1740 bp Sequence Blast
function
biosynthesis of menaquinone
product
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase
essential
yes
ec
2.2.1.9
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.8|Biosynthesis of menaquinone]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    3,150,563 → 3,152,305

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2 (according to Swiss-Prot)
  • Protein family

  • MenD subfamily (according to Swiss-Prot)
  • [SW|Cofactors]

  • thiamine pyrophosphate, divalent metal ion such as Mn(2+)) [Pubmed|20600129]
  • Structure

  • [PDB|2X7J] [Pubmed|20600129]
  • Expression and Regulation

    Operons

    genes
    [gene|8EFE4D2B07E54A0EE7D6821C9FEBFC7DC22FDE03|menF]-[gene|E3283812B7EB374EAC8722ECCE28432DE24B7B4C|menD]-[gene|74659B6794496912E139B3CF9EFF787B63729A3F|ytxM]-[gene|224584953B0BC1AF666CD69372B2D53AC56F6752|menB]-[gene|16412BAB04BBF9FD1C3653328923687874C81868|menE]-[gene|9B2F45BC37D4F356B366447A33DC95639EF484EE|menC]
    description
    [Pubmed|9387221,1629163,8550504]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8550504], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|mntA]' and '[protein|search|menC]' [PubMed|20525796]
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    References

  • 9387221,1629163,8550504,20600129