SubtiBank SubtiBank
cshA [Thu Dec 15 2016 14:10:23 GMT+0100 (CET)]
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cshA [Thu Dec 15 2016 14:10:23 GMT+0100 (CET)]

DEAD-box RNA helicase, important for adaptation to low temperatures
locus
BSU04580
pI
9.00
mw
57.00 kDa
function
RNA helicase
product
DEAD-box RNA helicase
essential
no
synonyms
ydbR

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.3|DEAD-box RNA helicases]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.5|Cold stress proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|DEAD-box RNA helicases], [SW|translation], [SW|cold stress proteins], [SW|membrane proteins]
  • Gene

    Coordinates on the chromosome (coding sequence)
    511,106 -> 512,641

    Phenotypes of a mutant

  • poor growth at low temperatures (16 to 20°C) [Pubmed|23175651]
  • reduced number of [SW|ribosome]s [Pubmed|23175651]
  • no expression of the ''[protein|search|frlB]-[protein|search|frlO]-[protein|search|frlN]-[protein|search|frlM]-[protein|search|frlD]'' operon [Pubmed|23175651]
  • strongly increased expression of the ''[protein|search|ysbA]-[protein|search|ysbB]'' operon [Pubmed|23175651]
  • transcription profile resulting from ''[protein|search|rny]'' depletion: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE36877 GEO] [Pubmed|23175651]
  • The protein

    Catalyzed reaction/ biological activity

  • RNA helicase
  • required for [SW|ribosome] assembly (biogenesis of the large subunit) [Pubmed|23175651]
  • Protein family

  • helicase C-terminal domain (according to Swiss-Prot) [SW|DEAD-box RNA helicases]
  • Paralogous protein(s)

  • [protein|search|CshB], [protein|search|DeaD], [protein|search|YfmL]
  • [SW|Localization]

  • cytoplasma, colocalizes with the ribosomes [Pubmed|16352840,27708634], cell membrane [Pubmed|20572937]
  • [SW|Interactions]

  • CshA interacts with the [SW|RNA polymerase] [Pubmed|21710567]
  • part of the [SW|RNA degradosome] [Pubmed|20572937]
  • [protein|search|CshA]-[protein|search|CshA] [Pubmed|20572937]
  • [protein|search|CshA]-[protein|search|CspB] [Pubmed|16352840]
  • [protein|search|CshA]-[protein|search|PnpA] [Pubmed|20572937]
  • [protein|search|CshA]-[protein|search|Rny] [Pubmed|20572937,21803996]
  • [protein|search|CshA]-[protein|search|RnjA] [Pubmed|20572937]
  • [protein|search|CshA]-[protein|search|Eno] [Pubmed|20572937]
  • [protein|search|CshA]-[protein|search|PfkA] [Pubmed|20572937]
  • [protein|search|DeaD]-[protein|search|CshA] [Pubmed|20572937]
  • [protein|search|RnpA]-[protein|search|CshA] [Pubmed|21764917]
  • [protein|search|RplA]-[protein|search|CshA] [Pubmed|23175651]
  • [protein|search|RplC]-[protein|search|CshA] [Pubmed|23175651]
  • Expression and Regulation

    Regulation

  • induced by cold shock [Pubmed|12399512]
  • constitutive, similar expression at high and low temperatures, throughout growth and in both minimal and complex medium [Pubmed|20572937]
  • Biological materials

    Mutant

  • GP1035 (aphA3), available in [SW|Jörg Stülke]'s lab
  • GP1083 (cat), available in [SW|Jörg Stülke]'s lab
  • Expression vector

  • for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], in [SW|pGP382]: pGP1387, available in [SW|Jörg Stülke]'s lab
  • for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from the native chromomsomal site: GP1026 (aphA3), available in [SW|Jörg Stülke]'s lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1386, available in [SW|Jörg Stülke]'s lab
  • GFP fusion

  • pGP1369 for chromosomal expression of CshA-YFP, available in [SW|Jörg Stülke]'s lab
  • ''B. subtilis'' GP1081 cshA-gfp spc, available in [SW|Jörg Stülke]'s lab,
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP1010 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • GP1074 (tet), available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]
  • References

  • 20572937,23175651,21803996,16352840,27708634,27965645