SubtiBank SubtiBank
citB [Tue Nov 22 2016 15:43:11 GMT+0100 (CET)]
Save the date! 20th International Conference on Bacilli and Gram-Positive Bacteria. Washington, D.C. July 23-26, 2019.

citB [Tue Nov 22 2016 15:43:11 GMT+0100 (CET)]

trigger enzyme: aconitase and RNA binding protein
locus
BSU18000
pI
4.00
mw
99.00 kDa
function
TCA cycle
product
trigger enzyme: aconitase
essential
no
ec
4.2.1.3
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.4|Trigger enzyme that acts by binding of a specific RNA element]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.4|RNA binding regulators]
  • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|trigger enzyme], [SW|RNA binding regulators], [SW|most abundant proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon], [SW|CcpC regulon], [SW|CodY regulon], [SW|FsrA regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,926,680 -> 1,929,409

    Phenotypes of a mutant

  • glutamate auxotrophy and a defect in sporulation [Pubmed|9393699] [SW|citZ]
  • The protein

    Catalyzed reaction/ biological activity

  • Citrate <=> isocitrate
  • Binding to iron responsive elements (IRE RNA) in the absence of the FeS cluster [Pubmed|23354745,10468622]
  • [SW|Cofactors]

  • FeS cluster
  • Structure

  • [PDB|1L5J] (''E. coli'')
  • [SW|Interactions]

  • [protein|search|Mdh]-[protein|search|CitB] [Pubmed|20933603]
  • [protein|search|CitG]-[protein|search|CitB] [Pubmed|20933603]
  • [protein|search|CitB]-[protein|search|SufU] (insertion of the Fe-S cluster) [Pubmed|21744456]
  • Additional information

  • ''B. subtilis'' aconitase is both an enzyme and an RNA binding protein ([SW|moonlighting protein]) [Pubmed|10468622]
  • extensive information on the structure and enzymatic properties of CitB can be found at [http://www.proteopedia.org/wiki/index.php/Aconitase Proteopedia]
  • Expression and Regulation

    Operon

  • ''[protein|search|citB]-[SW|yneN] '' [Pubmed|22383849]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|1310745]
  • Regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12591885]
  • repressed in the presence of glucose and glutamate ([protein|search|CcpC]) [Pubmed|10656796]
  • expressed upon transition into the stationary phase ([protein|search|AbrB]) [Pubmed|12591885], indirect negative regulation by [protein|search|AbrB] [Pubmed|20817675]
  • repressed by glucose (3.7-fold) ([protein|search|CcpA]) [Pubmed|12850135]
  • repression by glucose arginine ([protein|search|CcpC]) [Pubmed|16395550]
  • less expressed under conditions of extreme iron limitation ([protein|search|FsrA]) [Pubmed|18697947]
  • part of the iron sparing response ([protein|search|FsrA]) [Pubmed|22389480]
  • Regulatory mechanism

  • [protein|search|CodY]: transcription repression [Pubmed|12591885]
  • [protein|search|CcpC]: transcription repression (molecular inducer: citrate) [http://www.ncbi.nlm.nih.gov/sites/entrez/10656796 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/12100558 PubMed2]
  • [protein|search|CcpA]: transcription repression [Pubmed|12100558]
  • [protein|search|FsrA]: translational repression [Pubmed|18697947]
  • Additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Biological materials

    Mutant

  • GP1275 (''citB''::''erm''), available in [SW|Jörg Stülke]'s lab
  • GP1441 (spc), available in [SW|Jörg Stülke]'s lab
  • 1A999 (''citB''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A999&Search=1A999 BGSC]
  • GP2338 (''[protein|search|citB]''::''kan''), Cre-recombinase is integrated in ''[protein|search|sacA]'', available in [SW|Jörg Stülke]'s lab
  • GP2339 (''[protein|search|citB]''::''lox72''), Cre-recombinase is integrated in ''[protein|search|sacA]'', available in [SW|Jörg Stülke]'s lab
  • lacZ fusion

  • pGP700 (in [protein|search|pAC5]), available in [SW|Jörg Stülke]'s lab
  • GFP fusion

  • GP1434 (spc, based on [SW|pGP1870]), available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP1144 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • GP1145 (kan), available in [SW|Jörg Stülke]'s lab
  • Antibody

  • available in [SW|Linc Sonenshein]'s lab
  • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
  • [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • References

    Reviews

  • 12732309,2696478,18261896,18086213
  • Original publications

  • 18697947,20097860,2118511,12850135,2413006,10656796,10468622,6143742,16395550,16923907,9642180,9393699,12591885,2105305,20933603,21099137,21446632,21821766,22389480,23139400,23354745,20817675,15378759,16923908,21744456