SubtiBank SubtiBank
tsaE [Tue Oct 20 2015 15:46:58 GMT+0200 (CEST)]

tsaE [Tue Oct 20 2015 15:46:58 GMT+0200 (CEST)]

P-loop ATPase, required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA
locus
BSU05910
pI
4.00
mw
17.00 kDa
function
tRNA modification
product
tRNA modification enzyme
essential
no
synonyms
ydiB

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
  • [SW|Categories] containing this gene/protein

  • [SW|translation]
  • Gene

    Phenotypes of a mutant

  • reduced growth rate [Pubmed|19246765]
  • The protein

    Catalyzed reaction/ biological activity

  • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([protein|search|TsaB]-[protein|search|TsaD]-[protein|search|TsaE]) [Pubmed|23072323,21183954]
  • Protein family

  • UPF0079 family (according to Swiss-Prot)
  • Kinetic information

  • K(M) for ATP: 60 myM, V(max) 10 nmol/min [Pubmed|19246765]
  • [SW|Localization]

  • cytoplasm with the exception of the nucleoid region [Pubmed|19246765]
  • [SW|Interactions]

  • dimerizes and oligomerizes [Pubmed|19246765]
  • [protein|search|TsaB]-[protein|search|TsaE] [Pubmed|23072323]
  • [protein|search|TsaD]-[protein|search|TsaE] [Pubmed|23072323]
  • Additional information

  • ATPase activity is required for ''in vivo'' function of the protein [Pubmed|19246765]
  • Expression and Regulation

    Operon

  • ''[protein|search|thiL]-[protein|search|tsaE]-[protein|search|tsaB]-[protein|search|ydiD]-[protein|search|tsaD]'' [Pubmed|22383849]
  • Biological materials

    Expression vector

  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP833, available in [SW|Stülke] lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP841, available in [SW|Stülke] lab
  • References

  • 17005971,19246765,23072323,22383849